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Dynamic Alteration of the Colonic Microbiota in Intestinal Ischemia-Reperfusion Injury

Author

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  • Fan Wang
  • Qiurong Li
  • Chenyang Wang
  • Chun Tang
  • Jieshou Li

Abstract

Background: Intestinal ischemia-reperfusion (I/R) plays an important role in critical illnesses. Gut flora participate in the pathogenesis of the injury. This study is aimed at unraveling colonic microbiota alteration pattern and identifying specific bacterial species that differ significantly as well as observing colonic epithelium change in the same injury model during the reperfusion time course. Methodology/Principal Findings: Denaturing gradient gel electrophoresis (DGGE) was used to monitor the colonic microbiota of control rats and experimental rats that underwent 0.5 hour ischemia and 1, 3, 6, 12, 24, and 72 hours following reperfusion respectively. The microbiota similarity, bacterial diversity and species that characterized the dysbiosis were estimated based on the DGGE profiles using a combination of statistical approaches. The interested bacterial species in the gel were cut and sequenced and were subsequently quantified and confirmed with real-time PCR. Meanwhile, the epithelial barrier was checked by microscopy and D-lactate analysis. Colonic flora changed early and differed significantly at 6 hours after reperfusion and then started to recover. The shifts were characterized by the increase of Escherichia coli and Prevotella oralis, and Lactobacilli proliferation together with epithelia healing. Conclusion/Significance: This study shows for the first time that intestinal ischemia-reperfusion results in colonic flora dysbiosis that follows epithelia damage, and identifies the bacterial species that contribute most.

Suggested Citation

  • Fan Wang & Qiurong Li & Chenyang Wang & Chun Tang & Jieshou Li, 2012. "Dynamic Alteration of the Colonic Microbiota in Intestinal Ischemia-Reperfusion Injury," PLOS ONE, Public Library of Science, vol. 7(7), pages 1-9, July.
    • Handle: RePEc:plo:pone00:0042027
    DOI: 10.1371/journal.pone.0042027
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    1. Pirjo Wacklin & Harri Mäkivuokko & Noora Alakulppi & Janne Nikkilä & Heli Tenkanen & Jarkko Räbinä & Jukka Partanen & Kari Aranko & Jaana Mättö, 2011. "Secretor Genotype (FUT2 gene) Is Strongly Associated with the Composition of Bifidobacteria in the Human Intestine," PLOS ONE, Public Library of Science, vol. 6(5), pages 1-10, May.
    2. Arianna Nenci & Christoph Becker & Andy Wullaert & Ralph Gareus & Geert van Loo & Silvio Danese & Marion Huth & Alexei Nikolaev & Clemens Neufert & Blair Madison & Deborah Gumucio & Markus F. Neurath , 2007. "Epithelial NEMO links innate immunity to chronic intestinal inflammation," Nature, Nature, vol. 446(7135), pages 557-561, March.
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