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T7 RNA Polymerase Functions In Vitro without Clustering

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  • Kieran Finan
  • Joseph P Torella
  • Achillefs N Kapanidis
  • Peter R Cook

Abstract

Many nucleic acid polymerases function in clusters known as factories. We investigate whether the RNA polymerase (RNAP) of phage T7 also clusters when active. Using ‘pulldowns’ and fluorescence correlation spectroscopy we find that elongation complexes do not interact in vitro with a Kd

Suggested Citation

  • Kieran Finan & Joseph P Torella & Achillefs N Kapanidis & Peter R Cook, 2012. "T7 RNA Polymerase Functions In Vitro without Clustering," PLOS ONE, Public Library of Science, vol. 7(7), pages 1-9, July.
    • Handle: RePEc:plo:pone00:0040207
    DOI: 10.1371/journal.pone.0040207
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    References listed on IDEAS

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    1. Graham M. T. Cheetham & David Jeruzalmi & Thomas A Steitz, 1999. "Structural basis for initiation of transcription from an RNA polymerase–promoter complex," Nature, Nature, vol. 399(6731), pages 80-83, May.
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