IDEAS home Printed from https://ideas.repec.org/a/plo/pone00/0007706.html
   My bibliography  Save this article

Roles of ES Cell-Derived Gliogenic Neural Stem/Progenitor Cells in Functional Recovery after Spinal Cord Injury

Author

Listed:
  • Gentaro Kumagai
  • Yohei Okada
  • Junichi Yamane
  • Narihito Nagoshi
  • Kazuya Kitamura
  • Masahiko Mukaino
  • Osahiko Tsuji
  • Kanehiro Fujiyoshi
  • Hiroyuki Katoh
  • Seiji Okada
  • Shinsuke Shibata
  • Yumi Matsuzaki
  • Satoshi Toh
  • Yoshiaki Toyama
  • Masaya Nakamura
  • Hideyuki Okano

Abstract

Transplantation of neural stem/progenitor cells (NS/PCs) following the sub-acute phase of spinal cord injury (SCI) has been shown to promote functional recovery in rodent models. However, the types of cells most effective for treating SCI have not been clarified. Taking advantage of our recently established neurosphere-based culture system of ES cell-derived NS/PCs, in which primary neurospheres (PNS) and passaged secondary neurospheres (SNS) exhibit neurogenic and gliogenic potentials, respectively, here we examined the distinct effects of transplanting neurogenic and gliogenic NS/PCs on the functional recovery of a mouse model of SCI. ES cell-derived PNS and SNS transplanted 9 days after contusive injury at the Th10 level exhibited neurogenic and gliogenic differentiation tendencies, respectively, similar to those seen in vitro. Interestingly, transplantation of the gliogenic SNS, but not the neurogenic PNS, promoted axonal growth, remyelination, and angiogenesis, and resulted in significant locomotor functional recovery after SCI. These findings suggest that gliogenic NS/PCs are effective for promoting the recovery from SCI, and provide essential insight into the mechanisms through which cellular transplantation leads to functional improvement after SCI.

Suggested Citation

  • Gentaro Kumagai & Yohei Okada & Junichi Yamane & Narihito Nagoshi & Kazuya Kitamura & Masahiko Mukaino & Osahiko Tsuji & Kanehiro Fujiyoshi & Hiroyuki Katoh & Seiji Okada & Shinsuke Shibata & Yumi Mat, 2009. "Roles of ES Cell-Derived Gliogenic Neural Stem/Progenitor Cells in Functional Recovery after Spinal Cord Injury," PLOS ONE, Public Library of Science, vol. 4(11), pages 1-13, November.
    • Handle: RePEc:plo:pone00:0007706
    DOI: 10.1371/journal.pone.0007706
    as

    Download full text from publisher

    File URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0007706
    Download Restriction: no
    File URL: https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0007706&type=printable
    Download Restriction: no
    File URL: https://libkey.io/10.1371/journal.pone.0007706?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Sally Temple, 2001. "The development of neural stem cells," Nature, Nature, vol. 414(6859), pages 112-117, November.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Shunsuke Chuma & Kazuyuki Kiyosue & Taishu Akiyama & Masaki Kinoshita & Yukiho Shimazaki & Seiichi Uchiyama & Shingo Sotoma & Kohki Okabe & Yoshie Harada, 2024. "Implication of thermal signaling in neuronal differentiation revealed by manipulation and measurement of intracellular temperature," Nature Communications, Nature, vol. 15(1), pages 1-13, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:plo:pone00:0007706. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: plosone (email available below). General contact details of provider: https://journals.plos.org/plosone/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.