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Expression Profile of MicroRNAs in Young Stroke Patients

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  • Kay Sin Tan
  • Arunmozhiarasi Armugam
  • Sugunavathi Sepramaniam
  • Kai Ying Lim
  • Karolina Dwi Setyowati
  • Chee Woon Wang
  • Kandiah Jeyaseelan

Abstract

Background: The methods currently available for diagnosis and prognosis of cerebral ischaemia still require further improvements. Micro-RNAs (small non-coding RNAs) have been recently reported as useful biomarkers in diseases such as cancer and diabetes. We therefore carried out microRNA (miRNA) profiling from peripheral blood to detect and identify characteristic patterns in ischaemic stroke. Methods/Principal Findings: The ischaemic stroke patients aged between 18–49 years, characterized based on World Health Organization clinical criteria were further classified according to TOAST classification, a) Large-vessel atherosclerosis [n = 8] b) Small-vessel disease [n = 3] c) Cardioembolism [n = 5] d) Undetermined cause [n = 3]. The patients' functional status at the time of blood sampling (at the outpatient clinics) was evaluated with the modified Rankin Scale (mRS). Blood samples from normal (n = 5) individuals were used as controls. Total RNA extracted from whole blood was subjected to miroRNA profiling and real-time PCR analysis. Conclusion: We demonstrate that the peripheral blood miRNAs and their profiles can be developed as biomarkers in diagnosis and prognosis of cerebral ischaemic stroke. The dysregulated miRNAs have been detectable even after several months from the onset of stroke in what is usually regarded as neurologically stable patients.

Suggested Citation

  • Kay Sin Tan & Arunmozhiarasi Armugam & Sugunavathi Sepramaniam & Kai Ying Lim & Karolina Dwi Setyowati & Chee Woon Wang & Kandiah Jeyaseelan, 2009. "Expression Profile of MicroRNAs in Young Stroke Patients," PLOS ONE, Public Library of Science, vol. 4(11), pages 1-9, November.
    • Handle: RePEc:plo:pone00:0007689
    DOI: 10.1371/journal.pone.0007689
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    References listed on IDEAS

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    1. Jonathan B. Johnnidis & Marian H. Harris & Robert T. Wheeler & Sandra Stehling-Sun & Michael H. Lam & Oktay Kirak & Thijn R. Brummelkamp & Mark D. Fleming & Fernando D. Camargo, 2008. "Regulation of progenitor cell proliferation and granulocyte function by microRNA-223," Nature, Nature, vol. 451(7182), pages 1125-1129, February.
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