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Application of a household-based molecular xenomonitoring strategy to evaluate the lymphatic filariasis elimination program in Tamil Nadu, India

Author

Listed:
  • Swaminathan Subramanian
  • Purushothaman Jambulingam
  • Brian K Chu
  • Candasamy Sadanandane
  • Venkatesan Vasuki
  • Adinarayanan Srividya
  • Mohamed S Mohideen AbdulKader
  • Kaliannagounder Krishnamoorthy
  • Harikishan K Raju
  • Sandra J Laney
  • Steven A Williams
  • Ralph H Henderson

Abstract

Background: The monitoring and evaluation of lymphatic filariasis (LF) has largely relied on the detection of antigenemia and antibodies in human populations. Molecular xenomonitoring (MX), the detection of parasite DNA/RNA in mosquitoes, may be an effective complementary method, particularly for detecting signals in low-level prevalence areas where Culex is the primary mosquito vector. This paper investigated the application of a household-based sampling method for MX in Tamil Nadu, India. Methods: MX surveys were conducted in 2010 in two evaluation units (EUs): 1) a hotspot area, defined as sites with community microfilaria prevalence ≥1%, and 2) a larger area that also encompassed the hotspots. Households were systematically selected using a sampling interval proportional to the number of households in the EU. Mosquito pools were collected and analyzed by real-time polymerase chain reaction (qPCR). Two independent samples were taken in each EU to assess reproducibility of results. Follow-up surveys were conducted in 2012. Results: In 2010, the proportion of positive pools in the hotspot EU was 49.3% compared to 23.4% in the overall EU. In 2012, pool positivity was significantly reduced to 24.3% and 6.5%, respectively (p

Suggested Citation

  • Swaminathan Subramanian & Purushothaman Jambulingam & Brian K Chu & Candasamy Sadanandane & Venkatesan Vasuki & Adinarayanan Srividya & Mohamed S Mohideen AbdulKader & Kaliannagounder Krishnamoorthy &, 2017. "Application of a household-based molecular xenomonitoring strategy to evaluate the lymphatic filariasis elimination program in Tamil Nadu, India," PLOS Neglected Tropical Diseases, Public Library of Science, vol. 11(4), pages 1-15, April.
    • Handle: RePEc:plo:pntd00:0005519
    DOI: 10.1371/journal.pntd.0005519
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