A Sensitive and Specific Neural Signature for Picture-Induced Negative Affect

Neuroimaging has identified many correlates of emotion but has not yet yielded brain representations predictive of the intensity of emotional experiences in individuals. We used machine learning to identify a sensitive and specific signature of emotional responses to aversive images. This signature predicted the intensity of negative emotion in individual participants in cross validation (n =121) and test (n = 61) samples (high–low emotion = 93.5% accuracy). It was unresponsive to physical pain (emotion–pain = 92% discriminative accuracy), demonstrating that it is not a representation of generalized arousal or salience. The signature was comprised of mesoscale patterns spanning multiple cortical and subcortical systems, with no single system necessary or sufficient for predicting experience. Furthermore, it was not reducible to activity in traditional “emotion-related” regions (e.g., amygdala, insula) or resting-state networks (e.g., “salience,” “default mode”). Overall, this work identifies differentiable neural components of negative emotion and pain, providing a basis for new, brain-based taxonomies of affective processes.By using images to induce negative emotions in human participants, this study uses neuroimaging to develop and validate a distributed brain signature of emotion that can predict the magnitude and type of negative affective experience in new individuals.Author Summary: Emotions are an important aspect of human experience and behavior; yet, we do not have a clear understanding of how they are processed in the brain. We have identified a neural signature of negative emotion—a neural activation pattern distributed across the brain that accurately predicts how negative a person will feel after viewing an aversive image. This pattern encompasses multiple brain subnetworks in the cortex and subcortex. This neural activation pattern dramatically outperforms other brain indicators of emotion based on activation in individual regions (e.g., amygdala, insula, and anterior cingulate) as well as networks of regions (e.g., limbic and “salience” networks). In addition, no single subnetwork is necessary or sufficient for accurately determining the intensity and type of affective response. Finally, this pattern appears to be specific to picture-induced negative affect, as it did not respond to at least one other aversive experience: painful heat. Together, these results provide a neurophysiological marker for feelings induced by a widely used probe of negative affect and suggest that brain imaging has the potential to accurately uncover how someone is feeling based purely on measures of brain activity.