IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v635y2024i8037d10.1038_s41586-024-07979-9.html
   My bibliography  Save this article

Structural basis of archaeal FttA-dependent transcription termination

Author

Listed:
  • Linlin You

    (Rutgers University)

  • Chengyuan Wang

    (Rutgers University
    Chinese Academy of Sciences)

  • Vadim Molodtsov

    (Rutgers University
    Research Institute of Molecular and Cellular Medicine RUDN)

  • Konstantin Kuznedelov

    (Rutgers University)

  • Xinyi Miao

    (Rutgers University)

  • Breanna R. Wenck

    (Colorado State University)

  • Paul Ulisse

    (Colorado State University)

  • Travis J. Sanders

    (Colorado State University)

  • Craig J. Marshall

    (Colorado State University)

  • Emre Firlar

    (Rutgers University)

  • Jason T. Kaelber

    (Rutgers University)

  • Thomas J. Santangelo

    (Colorado State University)

  • Richard H. Ebright

    (Rutgers University)

Abstract

The ribonuclease FttA (also known as aCPSF and aCPSF1) mediates factor-dependent transcription termination in archaea1–3. Here we report the structure of a Thermococcus kodakarensis transcription pre-termination complex comprising FttA, Spt4, Spt5 and a transcription elongation complex (TEC). The structure shows that FttA interacts with the TEC in a manner that enables RNA to proceed directly from the TEC RNA-exit channel to the FttA catalytic centre and that enables endonucleolytic cleavage of RNA by FttA, followed by 5′→3′ exonucleolytic cleavage of RNA by FttA and concomitant 5′→3′ translocation of FttA on RNA, to apply mechanical force to the TEC and trigger termination. The structure further reveals that Spt5 bridges FttA and the TEC, explaining how Spt5 stimulates FttA-dependent termination. The results reveal functional analogy between bacterial and archaeal factor-dependent termination, functional homology between archaeal and eukaryotic factor-dependent termination, and fundamental mechanistic similarities in factor-dependent termination in bacteria, archaea, and eukaryotes.

Suggested Citation

  • Linlin You & Chengyuan Wang & Vadim Molodtsov & Konstantin Kuznedelov & Xinyi Miao & Breanna R. Wenck & Paul Ulisse & Travis J. Sanders & Craig J. Marshall & Emre Firlar & Jason T. Kaelber & Thomas J., 2024. "Structural basis of archaeal FttA-dependent transcription termination," Nature, Nature, vol. 635(8037), pages 229-236, November.
    • Handle: RePEc:nat:nature:v:635:y:2024:i:8037:d:10.1038_s41586-024-07979-9
    DOI: 10.1038/s41586-024-07979-9
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41586-024-07979-9
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/s41586-024-07979-9?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:635:y:2024:i:8037:d:10.1038_s41586-024-07979-9. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.