IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v617y2023i7961d10.1038_s41586-023-05981-1.html
   My bibliography  Save this article

Formin-mediated nuclear actin at androgen receptors promotes transcription

Author

Listed:
  • Julian Knerr

    (University of Freiburg)

  • Ralf Werner

    (University of Lübeck
    University of Lübeck)

  • Carsten Schwan

    (University of Freiburg)

  • Hong Wang

    (University of Freiburg)

  • Peter Gebhardt

    (University of Freiburg)

  • Helga Grötsch

    (University of Lübeck
    Veraxa Biotech)

  • Almuth Caliebe

    (University Hospital Schleswig-Holstein, University of Lübeck and Kiel University, Lübeck)

  • Malte Spielmann

    (University Hospital Schleswig-Holstein, University of Lübeck and Kiel University, Lübeck
    Partner site Hamburg, Lübeck)

  • Paul-Martin Holterhus

    (University Hospital of Schleswig-Holstein)

  • Robert Grosse

    (University of Freiburg
    University of Freiburg)

  • Nadine C. Hornig

    (University Hospital Schleswig-Holstein, University of Lübeck and Kiel University, Lübeck)

Abstract

Steroid hormone receptors are ligand-binding transcription factors essential for mammalian physiology. The androgen receptor (AR) binds androgens mediating gene expression for sexual, somatic and behavioural functions, and is involved in various conditions including androgen insensitivity syndrome and prostate cancer1. Here we identified functional mutations in the formin and actin nucleator DAAM2 in patients with androgen insensitivity syndrome. DAAM2 was enriched in the nucleus, where its localization correlated with that of the AR to form actin-dependent transcriptional droplets in response to dihydrotestosterone. DAAM2 AR droplets ranged from 0.02 to 0.06 µm3 in size and associated with active RNA polymerase II. DAAM2 polymerized actin directly at the AR to promote droplet coalescence in a highly dynamic manner, and nuclear actin polymerization is required for prostate-specific antigen expression in cancer cells. Our data uncover signal-regulated nuclear actin assembly at a steroid hormone receptor necessary for transcription.

Suggested Citation

  • Julian Knerr & Ralf Werner & Carsten Schwan & Hong Wang & Peter Gebhardt & Helga Grötsch & Almuth Caliebe & Malte Spielmann & Paul-Martin Holterhus & Robert Grosse & Nadine C. Hornig, 2023. "Formin-mediated nuclear actin at androgen receptors promotes transcription," Nature, Nature, vol. 617(7961), pages 616-622, May.
    • Handle: RePEc:nat:nature:v:617:y:2023:i:7961:d:10.1038_s41586-023-05981-1
    DOI: 10.1038/s41586-023-05981-1
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41586-023-05981-1
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/s41586-023-05981-1?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Maria Dilia Palumbieri & Chiara Merigliano & Daniel González-Acosta & Danina Kuster & Jana Krietsch & Henriette Stoy & Thomas Känel & Svenja Ulferts & Bettina Welter & Joël Frey & Cyril Doerdelmann & , 2023. "Nuclear actin polymerization rapidly mediates replication fork remodeling upon stress by limiting PrimPol activity," Nature Communications, Nature, vol. 14(1), pages 1-15, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:617:y:2023:i:7961:d:10.1038_s41586-023-05981-1. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.