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The Smc5/6 complex is a DNA loop-extruding motor

Author

Listed:
  • Biswajit Pradhan

    (Max Planck Institute of Biophysics)

  • Takaharu Kanno

    (Karolinska Institutet
    Karolinska Institutet)

  • Miki Umeda Igarashi

    (Karolinska Institutet
    Karolinska Institutet)

  • Mun Siong Loke

    (Max Planck Institute of Biophysics)

  • Martin Dieter Baaske

    (Max Planck Institute of Biophysics)

  • Jan Siu Kei Wong

    (Max Planck Institute of Biophysics)

  • Kristian Jeppsson

    (Karolinska Institutet
    Karolinska Institutet)

  • Camilla Björkegren

    (Karolinska Institutet
    Karolinska Institutet)

  • Eugene Kim

    (Max Planck Institute of Biophysics)

Abstract

Structural maintenance of chromosomes (SMC) protein complexes are essential for the spatial organization of chromosomes1. Whereas cohesin and condensin organize chromosomes by extrusion of DNA loops, the molecular functions of the third eukaryotic SMC complex, Smc5/6, remain largely unknown2. Using single-molecule imaging, we show that Smc5/6 forms DNA loops by extrusion. Upon ATP hydrolysis, Smc5/6 reels DNA symmetrically into loops at a force-dependent rate of one kilobase pair per second. Smc5/6 extrudes loops in the form of dimers, whereas monomeric Smc5/6 unidirectionally translocates along DNA. We also find that the subunits Nse5 and Nse6 (Nse5/6) act as negative regulators of loop extrusion. Nse5/6 inhibits loop-extrusion initiation by hindering Smc5/6 dimerization but has no influence on ongoing loop extrusion. Our findings reveal functions of Smc5/6 at the molecular level and establish DNA loop extrusion as a conserved mechanism among eukaryotic SMC complexes.

Suggested Citation

  • Biswajit Pradhan & Takaharu Kanno & Miki Umeda Igarashi & Mun Siong Loke & Martin Dieter Baaske & Jan Siu Kei Wong & Kristian Jeppsson & Camilla Björkegren & Eugene Kim, 2023. "The Smc5/6 complex is a DNA loop-extruding motor," Nature, Nature, vol. 616(7958), pages 843-848, April.
    • Handle: RePEc:nat:nature:v:616:y:2023:i:7958:d:10.1038_s41586-023-05963-3
    DOI: 10.1038/s41586-023-05963-3
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