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Generation of functional oocytes from male mice in vitro

Author

Listed:
  • Kenta Murakami

    (Kyushu University
    Kyushu University)

  • Nobuhiko Hamazaki

    (Kyushu University)

  • Norio Hamada

    (Kyushu University
    Kyushu University)

  • Go Nagamatsu

    (Kyushu University)

  • Ikuhiro Okamoto

    (Kyoto University
    Kyoto University)

  • Hiroshi Ohta

    (Kyoto University
    Kyoto University)

  • Yoshiaki Nosaka

    (Kyoto University
    Kyoto University)

  • Yukiko Ishikura

    (Kyoto University
    Kyoto University)

  • Tomoya S. Kitajima

    (RIKEN Center for Biosystems Dynamics Research)

  • Yuichiro Semba

    (Kyushu University)

  • Yuya Kunisaki

    (Kyushu University
    Kyushu University)

  • Fumio Arai

    (Kyushu University)

  • Koichi Akashi

    (Kyushu University)

  • Mitinori Saitou

    (Kyoto University
    Kyoto University
    Kyoto University)

  • Kiyoko Kato

    (Kyushu University)

  • Katsuhiko Hayashi

    (Kyushu University
    Osaka University
    Osaka University
    Osaka University)

Abstract

Sex chromosome disorders severely compromise gametogenesis in both males and females. In oogenesis, the presence of an additional Y chromosome or the loss of an X chromosome disturbs the robust production of oocytes1–5. Here we efficiently converted the XY chromosome set to XX without an additional Y chromosome in mouse pluripotent stem (PS) cells. In addition, this chromosomal alteration successfully eradicated trisomy 16, a model of Down’s syndrome, in PS cells. Artificially produced euploid XX PS cells differentiated into mature oocytes in culture with similar efficiency to native XX PS cells. Using this method, we differentiated induced pluripotent stem cells from the tail of a sexually mature male mouse into fully potent oocytes, which gave rise to offspring after fertilization. This study provides insights that could ameliorate infertility caused by sex chromosome or autosomal disorders, and opens the possibility of bipaternal reproduction.

Suggested Citation

  • Kenta Murakami & Nobuhiko Hamazaki & Norio Hamada & Go Nagamatsu & Ikuhiro Okamoto & Hiroshi Ohta & Yoshiaki Nosaka & Yukiko Ishikura & Tomoya S. Kitajima & Yuichiro Semba & Yuya Kunisaki & Fumio Arai, 2023. "Generation of functional oocytes from male mice in vitro," Nature, Nature, vol. 615(7954), pages 900-906, March.
    • Handle: RePEc:nat:nature:v:615:y:2023:i:7954:d:10.1038_s41586-023-05834-x
    DOI: 10.1038/s41586-023-05834-x
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