Author
Listed:
- Jinglei Zhai
(Chinese Academy of Sciences
Chinese Academy of Sciences
Beijing Institute for Stem Cell and Regenerative Medicine)
- Jing Guo
(Chinese Academy of Sciences
Chinese Academy of Sciences
Beijing Institute for Stem Cell and Regenerative Medicine
University of Chinese Academy of Sciences)
- Haifeng Wan
(Chinese Academy of Sciences
Chinese Academy of Sciences
Beijing Institute for Stem Cell and Regenerative Medicine)
- Luqing Qi
(Chinese Academy of Sciences
Chinese Academy of Sciences
Beijing Institute for Stem Cell and Regenerative Medicine
University of Chinese Academy of Sciences)
- Lizhong Liu
(University of Texas Southwestern Medical Center)
- Zhenyu Xiao
(Chinese Academy of Sciences
Chinese Academy of Sciences
Beijing Institute for Stem Cell and Regenerative Medicine)
- Long Yan
(Chinese Academy of Sciences
Chinese Academy of Sciences
Beijing Institute for Stem Cell and Regenerative Medicine)
- Daniel A. Schmitz
(University of Texas Southwestern Medical Center)
- Yanhong Xu
(Chinese Academy of Sciences
Chinese Academy of Sciences
Beijing Institute for Stem Cell and Regenerative Medicine
University of Chinese Academy of Sciences)
- Dainan Yu
(Chinese Academy of Sciences
Chinese Academy of Sciences
Beijing Institute for Stem Cell and Regenerative Medicine
University of Chinese Academy of Sciences)
- Xulun Wu
(Chinese Academy of Sciences
Chinese Academy of Sciences
Beijing Institute for Stem Cell and Regenerative Medicine
University of Chinese Academy of Sciences)
- Wentao Zhao
(Chinese Academy of Sciences
Chinese Academy of Sciences
Beijing Institute for Stem Cell and Regenerative Medicine
University of Chinese Academy of Sciences)
- Kunyuan Yu
(Chinese Academy of Sciences
Chinese Academy of Sciences
Beijing Institute for Stem Cell and Regenerative Medicine
University of Chinese Academy of Sciences)
- Xiangxiang Jiang
(Chinese Academy of Sciences
Anhui Medical University
the First Affiliated Hospital of Anhui Medical University)
- Fan Guo
(Chinese Academy of Sciences
Chinese Academy of Sciences
Beijing Institute for Stem Cell and Regenerative Medicine
University of Chinese Academy of Sciences)
- Jun Wu
(University of Texas Southwestern Medical Center
University of Texas Southwestern Medical Center
University of Texas Southwestern Medical Center)
- Hongmei Wang
(Chinese Academy of Sciences
Chinese Academy of Sciences
Beijing Institute for Stem Cell and Regenerative Medicine
University of Chinese Academy of Sciences)
Abstract
Our understanding of human early development is severely hampered by limited access to embryonic tissues. Due to their close evolutionary relationship with humans, nonhuman primates are often used as surrogates to understand human development but currently suffer from a lack of in vivo datasets, especially from gastrulation to early organogenesis during which the major embryonic cell types are dynamically specified. To fill this gap, we collected six Carnegie stage 8–11 cynomolgus monkey (Macaca fascicularis) embryos and performed in-depth transcriptomic analyses of 56,636 single cells. Our analyses show transcriptomic features of major perigastrulation cell types, which help shed light on morphogenetic events including primitive streak development, somitogenesis, gut tube formation, neural tube patterning and neural crest differentiation in primates. In addition, comparative analyses with mouse embryos and human embryoids uncovered conserved and divergent features of perigastrulation development across species—for example, species-specific dependency on Hippo signalling during presomitic mesoderm differentiation—and provide an initial assessment of relevant stem cell models of human early organogenesis. This comprehensive single-cell transcriptome atlas not only fills the knowledge gap in the nonhuman primate research field but also serves as an invaluable resource for understanding human embryogenesis and developmental disorders.
Suggested Citation
Jinglei Zhai & Jing Guo & Haifeng Wan & Luqing Qi & Lizhong Liu & Zhenyu Xiao & Long Yan & Daniel A. Schmitz & Yanhong Xu & Dainan Yu & Xulun Wu & Wentao Zhao & Kunyuan Yu & Xiangxiang Jiang & Fan Guo, 2022.
"Primate gastrulation and early organogenesis at single-cell resolution,"
Nature, Nature, vol. 612(7941), pages 732-738, December.
Handle:
RePEc:nat:nature:v:612:y:2022:i:7941:d:10.1038_s41586-022-05526-y
DOI: 10.1038/s41586-022-05526-y
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