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Adenylate cyclase activity of TIR1/AFB auxin receptors in plants

Author

Listed:
  • Linlin Qi

    (Institute of Science and Technology Austria (ISTA))

  • Mateusz Kwiatkowski

    (Nicolaus Copernicus University in Toruń)

  • Huihuang Chen

    (Institute of Science and Technology Austria (ISTA))

  • Lukas Hoermayer

    (Institute of Science and Technology Austria (ISTA))

  • Scott Sinclair

    (Institute of Science and Technology Austria (ISTA)
    Biosecurity New Zealand)

  • Minxia Zou

    (Institute of Science and Technology Austria (ISTA))

  • Charo I. Genio

    (Coventry University)

  • Martin F. Kubeš

    (University of Warwick)

  • Richard Napier

    (University of Warwick)

  • Krzysztof Jaworski

    (Nicolaus Copernicus University in Toruń)

  • Jiří Friml

    (Institute of Science and Technology Austria (ISTA))

Abstract

The phytohormone auxin is the major coordinative signal in plant development1, mediating transcriptional reprogramming by a well-established canonical signalling pathway. TRANSPORT INHIBITOR RESPONSE 1 (TIR1)/AUXIN-SIGNALING F-BOX (AFB) auxin receptors are F-box subunits of ubiquitin ligase complexes. In response to auxin, they associate with Aux/IAA transcriptional repressors and target them for degradation via ubiquitination2,3. Here we identify adenylate cyclase (AC) activity as an additional function of TIR1/AFB receptors across land plants. Auxin, together with Aux/IAAs, stimulates cAMP production. Three separate mutations in the AC motif of the TIR1 C-terminal region, all of which abolish the AC activity, each render TIR1 ineffective in mediating gravitropism and sustained auxin-induced root growth inhibition, and also affect auxin-induced transcriptional regulation. These results highlight the importance of TIR1/AFB AC activity in canonical auxin signalling. They also identify a unique phytohormone receptor cassette combining F-box and AC motifs, and the role of cAMP as a second messenger in plants.

Suggested Citation

  • Linlin Qi & Mateusz Kwiatkowski & Huihuang Chen & Lukas Hoermayer & Scott Sinclair & Minxia Zou & Charo I. Genio & Martin F. Kubeš & Richard Napier & Krzysztof Jaworski & Jiří Friml, 2022. "Adenylate cyclase activity of TIR1/AFB auxin receptors in plants," Nature, Nature, vol. 611(7934), pages 133-138, November.
    • Handle: RePEc:nat:nature:v:611:y:2022:i:7934:d:10.1038_s41586-022-05369-7
    DOI: 10.1038/s41586-022-05369-7
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