Author
Listed:
- Tomomasa Yokomizo
(Kumamoto University
Tokyo Women’s Medical University)
- Takako Ideue
(Kumamoto University)
- Saori Morino-Koga
(Kumamoto University)
- Cheng Yong Tham
(National University of Singapore)
- Tomohiko Sato
(The University of Tokyo)
- Naoki Takeda
(Kumamoto University)
- Yoshiaki Kubota
(Keio University School of Medicine)
- Mineo Kurokawa
(The University of Tokyo)
- Norio Komatsu
(Juntendo University Graduate School of Medicine)
- Minetaro Ogawa
(Kumamoto University)
- Kimi Araki
(Kumamoto University
Kumamoto University)
- Motomi Osato
(Kumamoto University
National University of Singapore
National University of Singapore)
- Toshio Suda
(Kumamoto University
National University of Singapore)
Abstract
Self-renewal and differentiation are tightly controlled to maintain haematopoietic stem cell (HSC) homeostasis in the adult bone marrow1,2. During fetal development, expansion of HSCs (self-renewal) and production of differentiated haematopoietic cells (differentiation) are both required to sustain the haematopoietic system for body growth3,4. However, it remains unclear how these two seemingly opposing tasks are accomplished within the short embryonic period. Here we used in vivo genetic tracing in mice to analyse the formation of HSCs and progenitors from intra-arterial haematopoietic clusters, which contain HSC precursors and express the transcription factor hepatic leukaemia factor (HLF). Through kinetic study, we observed the simultaneous formation of HSCs and defined progenitors—previously regarded as descendants of HSCs5—from the HLF+ precursor population, followed by prompt formation of the hierarchical haematopoietic population structure in the fetal liver in an HSC-independent manner. The transcription factor EVI1 is heterogeneously expressed within the precursor population, with EVI1hi cells being predominantly localized to intra-embryonic arteries and preferentially giving rise to HSCs. By genetically manipulating EVI1 expression, we were able to alter HSC and progenitor output from precursors in vivo. Using fate tracking, we also demonstrated that fetal HSCs are slowly used to produce short-term HSCs at late gestation. These data suggest that fetal HSCs minimally contribute to the generation of progenitors and functional blood cells before birth. Stem cell-independent pathways during development thus offer a rational strategy for the rapid and simultaneous growth of tissues and stem cell pools.
Suggested Citation
Tomomasa Yokomizo & Takako Ideue & Saori Morino-Koga & Cheng Yong Tham & Tomohiko Sato & Naoki Takeda & Yoshiaki Kubota & Mineo Kurokawa & Norio Komatsu & Minetaro Ogawa & Kimi Araki & Motomi Osato & , 2022.
"Independent origins of fetal liver haematopoietic stem and progenitor cells,"
Nature, Nature, vol. 609(7928), pages 779-784, September.
Handle:
RePEc:nat:nature:v:609:y:2022:i:7928:d:10.1038_s41586-022-05203-0
DOI: 10.1038/s41586-022-05203-0
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Citations
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Cited by:
- Ritsuko Nakai & Takafumi Yokota & Masahiro Tokunaga & Mikiro Takaishi & Tomomasa Yokomizo & Takao Sudo & Henyun Shi & Yoshiaki Yasumizu & Daisuke Okuzaki & Chikara Kokubu & Sachiyo Tanaka & Katsuyoshi, 2024.
"A newly identified gene Ahed plays essential roles in murine haematopoiesis,"
Nature Communications, Nature, vol. 15(1), pages 1-16, December.
- Roshana Thambyrajah & Maria Maqueda & Muhammad Zaki Fadlullah & Martin Proffitt & Wen Hao Neo & Yolanda Guillén & Marta Casado-Pelaez & Patricia Herrero-Molinero & Carla Brujas & Noemi Castelluccio & , 2024.
"IκBα controls dormancy in hematopoietic stem cells via retinoic acid during embryonic development,"
Nature Communications, Nature, vol. 15(1), pages 1-17, December.
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