Author
Listed:
- Allan-Hermann Pool
(California Institute of Technology)
- Tongtong Wang
(California Institute of Technology
Nankai University)
- David A. Stafford
(University of California, Berkeley)
- Rebecca K. Chance
(University of California, Berkeley)
- Sangjun Lee
(California Institute of Technology)
- John Ngai
(University of California, Berkeley
National Institutes of Health)
- Yuki Oka
(California Institute of Technology)
Abstract
Fluid intake is an essential innate behaviour that is mainly caused by two distinct types of thirst1–3. Increased blood osmolality induces osmotic thirst that drives animals to consume pure water. Conversely, the loss of body fluid induces hypovolaemic thirst, in which animals seek both water and minerals (salts) to recover blood volume. Circumventricular organs in the lamina terminalis are critical sites for sensing both types of thirst-inducing stimulus4–6. However, how different thirst modalities are encoded in the brain remains unknown. Here we employed stimulus-to-cell-type mapping using single-cell RNA sequencing to identify the cellular substrates that underlie distinct types of thirst. These studies revealed diverse types of excitatory and inhibitory neuron in each circumventricular organ structure. We show that unique combinations of these neuron types are activated under osmotic and hypovolaemic stresses. These results elucidate the cellular logic that underlies distinct thirst modalities. Furthermore, optogenetic gain of function in thirst-modality-specific cell types recapitulated water-specific and non-specific fluid appetite caused by the two distinct dipsogenic stimuli. Together, these results show that thirst is a multimodal physiological state, and that different thirst states are mediated by specific neuron types in the mammalian brain.
Suggested Citation
Allan-Hermann Pool & Tongtong Wang & David A. Stafford & Rebecca K. Chance & Sangjun Lee & John Ngai & Yuki Oka, 2020.
"The cellular basis of distinct thirst modalities,"
Nature, Nature, vol. 588(7836), pages 112-117, December.
Handle:
RePEc:nat:nature:v:588:y:2020:i:7836:d:10.1038_s41586-020-2821-8
DOI: 10.1038/s41586-020-2821-8
Download full text from publisher
As the access to this document is restricted, you may want to search for a different version of it.
Corrections
All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:588:y:2020:i:7836:d:10.1038_s41586-020-2821-8. See general information about how to correct material in RePEc.
If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.
We have no bibliographic references for this item. You can help adding them by using this form .
If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.
For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .
Please note that corrections may take a couple of weeks to filter through
the various RePEc services.