Author
Listed:
- Ioachim Pupeza
(Ludwig Maximilians University München
Max Planck Institute of Quantum Optics)
- Marinus Huber
(Ludwig Maximilians University München
Max Planck Institute of Quantum Optics)
- Michael Trubetskov
(Max Planck Institute of Quantum Optics)
- Wolfgang Schweinberger
(Ludwig Maximilians University München
King Saud University, Department of Physics and Astronomy)
- Syed A. Hussain
(Ludwig Maximilians University München
Max Planck Institute of Quantum Optics)
- Christina Hofer
(Ludwig Maximilians University München
Max Planck Institute of Quantum Optics)
- Kilian Fritsch
(Ludwig Maximilians University München)
- Markus Poetzlberger
(Max Planck Institute of Quantum Optics)
- Lenard Vamos
(Max Planck Institute of Quantum Optics)
- Ernst Fill
(Ludwig Maximilians University München)
- Tatiana Amotchkina
(Ludwig Maximilians University München)
- Kosmas V. Kepesidis
(Ludwig Maximilians University München)
- Alexander Apolonski
(Ludwig Maximilians University München)
- Nicholas Karpowicz
(Max Planck Institute of Quantum Optics)
- Vladimir Pervak
(Ludwig Maximilians University München
Max Planck Institute of Quantum Optics)
- Oleg Pronin
(Ludwig Maximilians University München
Max Planck Institute of Quantum Optics)
- Frank Fleischmann
(Max Planck Institute of Quantum Optics
Center for Molecular Fingerprinting)
- Abdallah Azzeer
(King Saud University, Department of Physics and Astronomy)
- Mihaela Žigman
(Ludwig Maximilians University München
Max Planck Institute of Quantum Optics
Center for Molecular Fingerprinting)
- Ferenc Krausz
(Ludwig Maximilians University München
Max Planck Institute of Quantum Optics
Center for Molecular Fingerprinting)
Abstract
The proper functioning of living systems and physiological phenotypes depends on molecular composition. Yet simultaneous quantitative detection of a wide variety of molecules remains a challenge1–8. Here we show how broadband optical coherence opens up opportunities for fingerprinting complex molecular ensembles in their natural environment. Vibrationally excited molecules emit a coherent electric field following few-cycle infrared laser excitation9–12, and this field is specific to the sample’s molecular composition. Employing electro-optic sampling10,12–15, we directly measure this global molecular fingerprint down to field strengths 107 times weaker than that of the excitation. This enables transillumination of intact living systems with thicknesses of the order of 0.1 millimetres, permitting broadband infrared spectroscopic probing of human cells and plant leaves. In a proof-of-concept analysis of human blood serum, temporal isolation of the infrared electric-field fingerprint from its excitation along with its sampling with attosecond timing precision results in detection sensitivity of submicrograms per millilitre of blood serum and a detectable dynamic range of molecular concentration exceeding 105. This technique promises improved molecular sensitivity and molecular coverage for probing complex, real-world biological and medical settings.
Suggested Citation
Ioachim Pupeza & Marinus Huber & Michael Trubetskov & Wolfgang Schweinberger & Syed A. Hussain & Christina Hofer & Kilian Fritsch & Markus Poetzlberger & Lenard Vamos & Ernst Fill & Tatiana Amotchkina, 2020.
"Field-resolved infrared spectroscopy of biological systems,"
Nature, Nature, vol. 577(7788), pages 52-59, January.
Handle:
RePEc:nat:nature:v:577:y:2020:i:7788:d:10.1038_s41586-019-1850-7
DOI: 10.1038/s41586-019-1850-7
Download full text from publisher
As the access to this document is restricted, you may want to search for a different version of it.
Citations
Citations are extracted by the
CitEc Project, subscribe to its
RSS feed for this item.
Cited by:
- Martin T. Peschel & Maximilian Högner & Theresa Buberl & Daniel Keefer & Regina Vivie-Riedle & Ioachim Pupeza, 2022.
"Sub-optical-cycle light-matter energy transfer in molecular vibrational spectroscopy,"
Nature Communications, Nature, vol. 13(1), pages 1-8, December.
- Enrico Ridente & Mikhail Mamaikin & Najd Altwaijry & Dmitry Zimin & Matthias F. Kling & Vladimir Pervak & Matthew Weidman & Ferenc Krausz & Nicholas Karpowicz, 2022.
"Electro-optic characterization of synthesized infrared-visible light fields,"
Nature Communications, Nature, vol. 13(1), pages 1-7, December.
- Mingchen Liu & Robert M. Gray & Luis Costa & Charles R. Markus & Arkadev Roy & Alireza Marandi, 2023.
"Mid-infrared cross-comb spectroscopy,"
Nature Communications, Nature, vol. 14(1), pages 1-8, December.
Corrections
All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:577:y:2020:i:7788:d:10.1038_s41586-019-1850-7. See general information about how to correct material in RePEc.
If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.
We have no bibliographic references for this item. You can help adding them by using this form .
If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.
For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .
Please note that corrections may take a couple of weeks to filter through
the various RePEc services.