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Force-induced conformational changes in PIEZO1

Author

Listed:
  • Yi-Chih Lin

    (Weill Cornell Medicine
    Weill Cornell Medicine)

  • Yusong R. Guo

    (TheRockefeller University)

  • Atsushi Miyagi

    (Weill Cornell Medicine
    Weill Cornell Medicine)

  • Jesper Levring

    (TheRockefeller University)

  • Roderick MacKinnon

    (TheRockefeller University)

  • Simon Scheuring

    (Weill Cornell Medicine
    Weill Cornell Medicine)

Abstract

PIEZO1 is a mechanosensitive channel that converts applied force into electrical signals. Partial molecular structures show that PIEZO1 is a bowl-shaped trimer with extended arms. Here we use cryo-electron microscopy to show that PIEZO1 adopts different degrees of curvature in lipid vesicles of different sizes. We also use high-speed atomic force microscopy to analyse the deformability of PIEZO1 under force in membranes on a mica surface, and show that PIEZO1 can be flattened reversibly into the membrane plane. By approximating the absolute force applied, we estimate a range of values for the mechanical spring constant of PIEZO1. Both methods of microscopy demonstrate that PIEZO1 can deform its shape towards a planar structure. This deformation could explain how lateral membrane tension can be converted into a conformation-dependent change in free energy to gate the PIEZO1 channel in response to mechanical perturbations.

Suggested Citation

  • Yi-Chih Lin & Yusong R. Guo & Atsushi Miyagi & Jesper Levring & Roderick MacKinnon & Simon Scheuring, 2019. "Force-induced conformational changes in PIEZO1," Nature, Nature, vol. 573(7773), pages 230-234, September.
    • Handle: RePEc:nat:nature:v:573:y:2019:i:7773:d:10.1038_s41586-019-1499-2
    DOI: 10.1038/s41586-019-1499-2
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    Cited by:

    1. Jingying Zhang & Grigory Maksaev & Peng Yuan, 2023. "Open structure and gating of the Arabidopsis mechanosensitive ion channel MSL10," Nature Communications, Nature, vol. 14(1), pages 1-9, December.

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