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The atomic structure of a eukaryotic oligosaccharyltransferase complex

Author

Listed:
  • Lin Bai

    (Center for Epigenetics, Van Andel Research Institute)

  • Tong Wang

    (Advanced Science Research Center at the Graduate Center of the City University of New York)

  • Gongpu Zhao

    (David Van Andel Advanced Cryo-Electron Microscopy Suite, Van Andel Research Institute)

  • Amanda Kovach

    (Center for Epigenetics, Van Andel Research Institute)

  • Huilin Li

    (Center for Epigenetics, Van Andel Research Institute)

Abstract

N-glycosylation is a ubiquitous modification of eukaryotic secretory and membrane-bound proteins; about 90% of glycoproteins are N-glycosylated. The reaction is catalysed by an eight-protein oligosaccharyltransferase (OST) complex that is embedded in the endoplasmic reticulum membrane. Our understanding of eukaryotic protein N-glycosylation has been limited owing to the lack of high-resolution structures. Here we report a 3.5 Å resolution cryo-electron microscopy structure of the Saccharomyces cerevisiae OST complex, revealing the structures of subunits Ost1–Ost5, Stt3, Wbp1 and Swp1. We found that seven phospholipids mediate many of the inter-subunit interactions, and an Stt3 N-glycan mediates interactions with Wbp1 and Swp1 in the lumen. Ost3 was found to mediate the OST–Sec61 translocon interface, funnelling the acceptor peptide towards the OST catalytic site as the nascent peptide emerges from the translocon. The structure provides insights into co-translational protein N-glycosylation, and may facilitate the development of small-molecule inhibitors that target this process.

Suggested Citation

  • Lin Bai & Tong Wang & Gongpu Zhao & Amanda Kovach & Huilin Li, 2018. "The atomic structure of a eukaryotic oligosaccharyltransferase complex," Nature, Nature, vol. 555(7696), pages 328-333, March.
    • Handle: RePEc:nat:nature:v:555:y:2018:i:7696:d:10.1038_nature25755
    DOI: 10.1038/nature25755
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    Cited by:

    1. Ana S. Ramírez & Mario Capitani & Giorgio Pesciullesi & Julia Kowal & Joël S. Bloch & Rossitza N. Irobalieva & Jean-Louis Reymond & Markus Aebi & Kaspar P. Locher, 2022. "Molecular basis for glycan recognition and reaction priming of eukaryotic oligosaccharyltransferase," Nature Communications, Nature, vol. 13(1), pages 1-12, December.

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