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Prolonged Mek1/2 suppression impairs the developmental potential of embryonic stem cells

Author

Listed:
  • Jiho Choi

    (Massachusetts General Hospital Department of Molecular Biology
    Massachusetts General Hospital Cancer Center and Center for Regenerative Medicine
    Harvard University
    Harvard Stem Cell Institute, 1350 Massachusetts Avenue)

  • Aaron J. Huebner

    (Massachusetts General Hospital Department of Molecular Biology
    Massachusetts General Hospital Cancer Center and Center for Regenerative Medicine
    Harvard University
    Harvard Stem Cell Institute, 1350 Massachusetts Avenue)

  • Kendell Clement

    (Harvard University
    Harvard Stem Cell Institute, 1350 Massachusetts Avenue
    Broad Institute of MIT and Harvard)

  • Ryan M. Walsh

    (Massachusetts General Hospital Department of Molecular Biology
    Massachusetts General Hospital Cancer Center and Center for Regenerative Medicine
    Harvard University
    Harvard Stem Cell Institute, 1350 Massachusetts Avenue)

  • Andrej Savol

    (Massachusetts General Hospital Department of Molecular Biology)

  • Kaixuan Lin

    (Yale University School of Medicine)

  • Hongcang Gu

    (Broad Institute of MIT and Harvard)

  • Bruno Di Stefano

    (Massachusetts General Hospital Department of Molecular Biology
    Massachusetts General Hospital Cancer Center and Center for Regenerative Medicine
    Harvard University
    Harvard Stem Cell Institute, 1350 Massachusetts Avenue)

  • Justin Brumbaugh

    (Massachusetts General Hospital Department of Molecular Biology
    Massachusetts General Hospital Cancer Center and Center for Regenerative Medicine
    Harvard University
    Harvard Stem Cell Institute, 1350 Massachusetts Avenue)

  • Sang-Yong Kim

    (New York University Langone Medical Center)

  • Jafar Sharif

    (Center for Integrative Medical Sciences, RIKEN National Research and Development Agency, 1-7-22 Suehiuro-cho, Tsurumi-ku)

  • Christopher M. Rose

    (Harvard Medical School)

  • Arman Mohammad

    (Broad Institute of MIT and Harvard)

  • Junko Odajima

    (Massachusetts General Hospital Cancer Center and Center for Regenerative Medicine)

  • Jean Charron

    (Centre de recherche sur le cancer de l’Université Laval, CRCHU de Québec, L’Hôtel-Dieu de Québec, 9, rue McMahon)

  • Toshi Shioda

    (Massachusetts General Hospital Cancer Center and Center for Regenerative Medicine)

  • Andreas Gnirke

    (Broad Institute of MIT and Harvard)

  • Steven Gygi

    (Harvard Medical School)

  • Haruhiko Koseki

    (Center for Integrative Medical Sciences, RIKEN National Research and Development Agency, 1-7-22 Suehiuro-cho, Tsurumi-ku)

  • Ruslan I. Sadreyev

    (Massachusetts General Hospital Department of Molecular Biology)

  • Andrew Xiao

    (Yale University School of Medicine)

  • Alexander Meissner

    (Harvard University
    Harvard Stem Cell Institute, 1350 Massachusetts Avenue
    Broad Institute of MIT and Harvard)

  • Konrad Hochedlinger

    (Massachusetts General Hospital Department of Molecular Biology
    Massachusetts General Hospital Cancer Center and Center for Regenerative Medicine
    Harvard University
    Harvard Stem Cell Institute, 1350 Massachusetts Avenue)

Abstract

Long-term culture of male embryonic stem cells in naive conditions containing Mek1/2 and Gsk3a/b inhibitors leads to irreversible changes in epigenetic and genomic stability that compromise their in vivo developmental potential.

Suggested Citation

  • Jiho Choi & Aaron J. Huebner & Kendell Clement & Ryan M. Walsh & Andrej Savol & Kaixuan Lin & Hongcang Gu & Bruno Di Stefano & Justin Brumbaugh & Sang-Yong Kim & Jafar Sharif & Christopher M. Rose & A, 2017. "Prolonged Mek1/2 suppression impairs the developmental potential of embryonic stem cells," Nature, Nature, vol. 548(7666), pages 219-223, August.
    • Handle: RePEc:nat:nature:v:548:y:2017:i:7666:d:10.1038_nature23274
    DOI: 10.1038/nature23274
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    Cited by:

    1. Maria Arez & Melanie Eckersley-Maslin & Tajda Klobučar & João Gilsa Lopes & Felix Krueger & Annalisa Mupo & Ana Cláudia Raposo & David Oxley & Samantha Mancino & Anne-Valerie Gendrel & Bruno Bernardes, 2022. "Imprinting fidelity in mouse iPSCs depends on sex of donor cell and medium formulation," Nature Communications, Nature, vol. 13(1), pages 1-20, December.
    2. Andrew Keniry & Natasha Jansz & Linden J. Gearing & Iromi Wanigasuriya & Joseph Chen & Christian M. Nefzger & Peter F. Hickey & Quentin Gouil & Joy Liu & Kelsey A. Breslin & Megan Iminitoff & Tamara B, 2022. "BAF complex-mediated chromatin relaxation is required for establishment of X chromosome inactivation," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    3. Shiran Bar & Dan Vershkov & Gal Keshet & Elyad Lezmi & Naama Meller & Atilgan Yilmaz & Ofra Yanuka & Malka Nissim-Rafinia & Eran Meshorer & Talia Eldar-Geva & Nissim Benvenisty, 2021. "Identifying regulators of parental imprinting by CRISPR/Cas9 screening in haploid human embryonic stem cells," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    4. Kentaro Mochizuki & Jafar Sharif & Kenjiro Shirane & Kousuke Uranishi & Aaron B. Bogutz & Sanne M. Janssen & Ayumu Suzuki & Akihiko Okuda & Haruhiko Koseki & Matthew C. Lorincz, 2021. "Repression of germline genes by PRC1.6 and SETDB1 in the early embryo precedes DNA methylation-mediated silencing," Nature Communications, Nature, vol. 12(1), pages 1-15, December.
    5. Sandra Rogala & Tamer Ali & Maria-Theodora Melissari & Sandra Währisch & Peggy Schuster & Alexandre Sarre & Rebeca Cordellini Emídio & Thomas Boettger & Eva-Maria Rogg & Jaskiran Kaur & Jaya Krishnan , 2023. "The lncRNA Sweetheart regulates compensatory cardiac hypertrophy after myocardial injury in murine males," Nature Communications, Nature, vol. 14(1), pages 1-14, December.

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