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Structural basis of CRISPR–SpyCas9 inhibition by an anti-CRISPR protein

Author

Listed:
  • De Dong

    (HIT Center for Life Sciences, School of Life Science and Technology, Harbin Institute of Technology)

  • Minghui Guo

    (HIT Center for Life Sciences, School of Life Science and Technology, Harbin Institute of Technology)

  • Sihan Wang

    (HIT Center for Life Sciences, School of Life Science and Technology, Harbin Institute of Technology)

  • Yuwei Zhu

    (HIT Center for Life Sciences, School of Life Science and Technology, Harbin Institute of Technology)

  • Shuo Wang

    (HIT Center for Life Sciences, School of Life Science and Technology, Harbin Institute of Technology)

  • Zhi Xiong

    (HIT Center for Life Sciences, School of Life Science and Technology, Harbin Institute of Technology)

  • Jianzheng Yang

    (HIT Center for Life Sciences, School of Life Science and Technology, Harbin Institute of Technology)

  • Zengliang Xu

    (HIT Center for Life Sciences, School of Life Science and Technology, Harbin Institute of Technology)

  • Zhiwei Huang

    (HIT Center for Life Sciences, School of Life Science and Technology, Harbin Institute of Technology)

Abstract

The structure of the anti-CRISPR protein AcrIIA4, in complex with a single-guide RNA and Cas9, reveals that the protein inhibits DNA binding and blocks the Cas9 endonuclease active site.

Suggested Citation

  • De Dong & Minghui Guo & Sihan Wang & Yuwei Zhu & Shuo Wang & Zhi Xiong & Jianzheng Yang & Zengliang Xu & Zhiwei Huang, 2017. "Structural basis of CRISPR–SpyCas9 inhibition by an anti-CRISPR protein," Nature, Nature, vol. 546(7658), pages 436-439, June.
    • Handle: RePEc:nat:nature:v:546:y:2017:i:7658:d:10.1038_nature22377
    DOI: 10.1038/nature22377
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    Cited by:

    1. Yanan Zhao & Jiaojiao Hu & Shan-Shan Yang & Jing Zhong & Jianping Liu & Shuo Wang & Yuzhuo Jiao & Fang Jiang & Ruiyang Zhai & Bingnan Ren & Hua Cong & Yuwei Zhu & Fengtong Han & Jixian Zhang & Yue Xu , 2022. "A redox switch regulates the assembly and anti-CRISPR activity of AcrIIC1," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    2. Xieshuting Deng & Wei Sun & Xueyan Li & Jiuyu Wang & Zhi Cheng & Gang Sheng & Yanli Wang, 2024. "An anti-CRISPR that represses its own transcription while blocking Cas9-target DNA binding," Nature Communications, Nature, vol. 15(1), pages 1-13, December.

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