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In vitro and ex vivo strategies for intracellular delivery

Author

Listed:
  • Martin P. Stewart

    (Massachusetts Institute of Technology
    The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology)

  • Armon Sharei

    (Massachusetts Institute of Technology
    The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology
    Harvard Medical School
    The Ragon Institute of MGH, MIT and Harvard)

  • Xiaoyun Ding

    (Massachusetts Institute of Technology
    The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology)

  • Gaurav Sahay

    (Collaborative Life Science Building, College of Pharmacy, Oregon State University)

  • Robert Langer

    (Massachusetts Institute of Technology
    The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology)

  • Klavs F. Jensen

    (Massachusetts Institute of Technology)

Abstract

Intracellular delivery of materials has become a critical component of genome-editing approaches, ex vivo cell-based therapies, and a diversity of fundamental research applications. Limitations of current technologies motivate development of next-generation systems that can deliver a broad variety of cargo to diverse cell types. Here we review in vitro and ex vivo intracellular delivery approaches with a focus on mechanisms, challenges and opportunities. In particular, we emphasize membrane-disruption-based delivery methods and the transformative role of nanotechnology, microfluidics and laboratory-on-chip technology in advancing the field.

Suggested Citation

  • Martin P. Stewart & Armon Sharei & Xiaoyun Ding & Gaurav Sahay & Robert Langer & Klavs F. Jensen, 2016. "In vitro and ex vivo strategies for intracellular delivery," Nature, Nature, vol. 538(7624), pages 183-192, October.
    • Handle: RePEc:nat:nature:v:538:y:2016:i:7624:d:10.1038_nature19764
    DOI: 10.1038/nature19764
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    Cited by:

    1. Jingen Zhu & Himanshu Batra & Neeti Ananthaswamy & Marthandan Mahalingam & Pan Tao & Xiaorong Wu & Wenzheng Guo & Andrei Fokine & Venigalla B. Rao, 2023. "Design of bacteriophage T4-based artificial viral vectors for human genome remodeling," Nature Communications, Nature, vol. 14(1), pages 1-19, December.
    2. Huimin Kong & Chunxiong Zheng & Ke Yi & Rachel L. Mintz & Yeh-Hsing Lao & Yu Tao & Mingqiang Li, 2024. "An antifouling membrane-fusogenic liposome for effective intracellular delivery in vivo," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    3. Erwin De Genst & Kylie S. Foo & Yao Xiao & Eduarde Rohner & Emma de Vries & Jesper Sohlmér & Nevin Witman & Alejandro Hidalgo & Terje R. S. Kolstad & William E. Louch & Susanne Pehrsson & Andrew Park , 2022. "Blocking phospholamban with VHH intrabodies enhances contractility and relaxation in heart failure," Nature Communications, Nature, vol. 13(1), pages 1-18, December.

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