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Early myeloid lineage choice is not initiated by random PU.1 to GATA1 protein ratios

Author

Listed:
  • Philipp S. Hoppe

    (Department of Biosystems Science and Engineering
    Research Unit Stem Cell Dynamics, Helmholtz Zentrum München)

  • Michael Schwarzfischer

    (Institute of Computational Biology, Helmholtz Zentrum München)

  • Dirk Loeffler

    (Department of Biosystems Science and Engineering
    Research Unit Stem Cell Dynamics, Helmholtz Zentrum München)

  • Konstantinos D. Kokkaliaris

    (Department of Biosystems Science and Engineering
    Research Unit Stem Cell Dynamics, Helmholtz Zentrum München)

  • Oliver Hilsenbeck

    (Department of Biosystems Science and Engineering
    Research Unit Stem Cell Dynamics, Helmholtz Zentrum München
    Institute of Computational Biology, Helmholtz Zentrum München)

  • Nadine Moritz

    (Research Unit Stem Cell Dynamics, Helmholtz Zentrum München)

  • Max Endele

    (Department of Biosystems Science and Engineering
    Research Unit Stem Cell Dynamics, Helmholtz Zentrum München)

  • Adam Filipczyk

    (Research Unit Stem Cell Dynamics, Helmholtz Zentrum München)

  • Adriana Gambardella

    (MRC Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, University of Oxford)

  • Nouraiz Ahmed

    (Department of Biosystems Science and Engineering)

  • Martin Etzrodt

    (Department of Biosystems Science and Engineering)

  • Daniel L. Coutu

    (Department of Biosystems Science and Engineering)

  • Michael A. Rieger

    (Research Unit Stem Cell Dynamics, Helmholtz Zentrum München)

  • Carsten Marr

    (Institute of Computational Biology, Helmholtz Zentrum München)

  • Michael K. Strasser

    (Institute of Computational Biology, Helmholtz Zentrum München)

  • Bernhard Schauberger

    (Research Unit Stem Cell Dynamics, Helmholtz Zentrum München)

  • Ingo Burtscher

    (Institute of Diabetes and Regeneration Research, Institute of Stem Cell Research, Helmholtz Zentrum München)

  • Olga Ermakova

    (Istituto di Biologia Cellulare e Neurobiologia, CNR)

  • Antje Bürger

    (Institute of Developmental Genetics, Helmholtz Zentrum München)

  • Heiko Lickert

    (Institute of Diabetes and Regeneration Research, Institute of Stem Cell Research, Helmholtz Zentrum München
    Beta Cell Biology, Medical Faculty, Technical University Munich (TUM))

  • Claus Nerlov

    (MRC Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, University of Oxford
    EMBL Mouse Biology Unit)

  • Fabian J. Theis

    (Institute of Computational Biology, Helmholtz Zentrum München
    Technical University Munich)

  • Timm Schroeder

    (Department of Biosystems Science and Engineering
    Research Unit Stem Cell Dynamics, Helmholtz Zentrum München)

Abstract

Live imaging and single-cell analyses are used to show that decision-making by differentiating haematopoietic stem cells between the megakaryocytic–erythroid and granulocytic–monocytic lineages is not initiated by stochastic switching between the lineage-specific transcription factors PU.1 and GATA1, which challenges the previous model of early myeloid lineage choice.

Suggested Citation

  • Philipp S. Hoppe & Michael Schwarzfischer & Dirk Loeffler & Konstantinos D. Kokkaliaris & Oliver Hilsenbeck & Nadine Moritz & Max Endele & Adam Filipczyk & Adriana Gambardella & Nouraiz Ahmed & Martin, 2016. "Early myeloid lineage choice is not initiated by random PU.1 to GATA1 protein ratios," Nature, Nature, vol. 535(7611), pages 299-302, July.
    • Handle: RePEc:nat:nature:v:535:y:2016:i:7611:d:10.1038_nature18320
    DOI: 10.1038/nature18320
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    Cited by:

    1. Philip Dettinger & Tobias Kull & Geethika Arekatla & Nouraiz Ahmed & Yang Zhang & Florin Schneiter & Arne Wehling & Daniel Schirmacher & Shunsuke Kawamura & Dirk Loeffler & Timm Schroeder, 2022. "Open-source personal pipetting robots with live-cell incubation and microscopy compatibility," Nature Communications, Nature, vol. 13(1), pages 1-12, December.

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