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Dissecting direct reprogramming from fibroblast to neuron using single-cell RNA-seq

Author

Listed:
  • Barbara Treutlein

    (Stanford University
    Max Planck Institute for Evolutionary Anthropology)

  • Qian Yi Lee

    (Stanford University
    Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine
    Stanford University School of Medicine)

  • J. Gray Camp

    (Stanford University School of Medicine)

  • Moritz Mall

    (Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine
    Stanford University School of Medicine)

  • Winston Koh

    (Stanford University)

  • Seyed Ali Mohammad Shariati

    (Stanford University)

  • Sopheak Sim

    (Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine)

  • Norma F. Neff

    (Stanford University)

  • Jan M. Skotheim

    (Stanford University)

  • Marius Wernig

    (Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine
    Stanford University School of Medicine)

  • Stephen R. Quake

    (Stanford University
    Howard Hughes Medical Institute)

Abstract

The transcriptome changes driving the conversion of fibroblasts to neurons at the single-cell level are reported, revealing that early neuronal reprogramming steps are homogenous, driven by the proneural pioneer factor Ascl1; the expression of myogenic genes then has a dampening effect on efficiency, which needs to be counteracted by the neuronal factors Myt1l and Brn2 for more efficient reprogramming.

Suggested Citation

  • Barbara Treutlein & Qian Yi Lee & J. Gray Camp & Moritz Mall & Winston Koh & Seyed Ali Mohammad Shariati & Sopheak Sim & Norma F. Neff & Jan M. Skotheim & Marius Wernig & Stephen R. Quake, 2016. "Dissecting direct reprogramming from fibroblast to neuron using single-cell RNA-seq," Nature, Nature, vol. 534(7607), pages 391-395, June.
    • Handle: RePEc:nat:nature:v:534:y:2016:i:7607:d:10.1038_nature18323
    DOI: 10.1038/nature18323
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    Cited by:

    1. Angélique Richard & Loïs Boullu & Ulysse Herbach & Arnaud Bonnafoux & Valérie Morin & Elodie Vallin & Anissa Guillemin & Nan Papili Gao & Rudiyanto Gunawan & Jérémie Cosette & Ophélie Arnaud & Jean-Ja, 2016. "Single-Cell-Based Analysis Highlights a Surge in Cell-to-Cell Molecular Variability Preceding Irreversible Commitment in a Differentiation Process," PLOS Biology, Public Library of Science, vol. 14(12), pages 1-35, December.
    2. Gintautas Vainorius & Maria Novatchkova & Georg Michlits & Juliane Christina Baar & Cecilia Raupach & Joonsun Lee & Ramesh Yelagandula & Marius Wernig & Ulrich Elling, 2023. "Ascl1 and Ngn2 convert mouse embryonic stem cells to neurons via functionally distinct paths," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    3. Chieh Lin & Jun Ding & Ziv Bar-Joseph, 2020. "Inferring TF activation order in time series scRNA-Seq studies," PLOS Computational Biology, Public Library of Science, vol. 16(2), pages 1-19, February.

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