IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v533y2016i7603d10.1038_nature17970.html
   My bibliography  Save this article

Near-atomic resolution visualization of human transcription promoter opening

Author

Listed:
  • Yuan He

    (Lawrence Berkeley National Laboratory
    Northwestern University)

  • Chunli Yan

    (Center for Diagnostics and Therapeutics, Georgia State University)

  • Jie Fang

    (Howard Hughes Medical Institute, University of California)

  • Carla Inouye

    (Li Ka Shing Center for Biomedical and Health Sciences, University of California)

  • Robert Tjian

    (Howard Hughes Medical Institute, University of California
    Li Ka Shing Center for Biomedical and Health Sciences, University of California
    University of California)

  • Ivaylo Ivanov

    (Center for Diagnostics and Therapeutics, Georgia State University)

  • Eva Nogales

    (Lawrence Berkeley National Laboratory
    Howard Hughes Medical Institute, University of California
    University of California)

Abstract

In eukaryotic transcription initiation, a large multi-subunit pre-initiation complex (PIC) that assembles at the core promoter is required for the opening of the duplex DNA and identification of the start site for transcription by RNA polymerase II. Here we use cryo-electron microscropy (cryo-EM) to determine near-atomic resolution structures of the human PIC in a closed state (engaged with duplex DNA), an open state (engaged with a transcription bubble), and an initially transcribing complex (containing six base pairs of DNA–RNA hybrid). Our studies provide structures for previously uncharacterized components of the PIC, such as TFIIE and TFIIH, and segments of TFIIA, TFIIB and TFIIF. Comparison of the different structures reveals the sequential conformational changes that accompany the transition from each state to the next throughout the transcription initiation process. This analysis illustrates the key role of TFIIB in transcription bubble stabilization and provides strong structural support for a translocase activity of XPB.

Suggested Citation

  • Yuan He & Chunli Yan & Jie Fang & Carla Inouye & Robert Tjian & Ivaylo Ivanov & Eva Nogales, 2016. "Near-atomic resolution visualization of human transcription promoter opening," Nature, Nature, vol. 533(7603), pages 359-365, May.
    • Handle: RePEc:nat:nature:v:533:y:2016:i:7603:d:10.1038_nature17970
    DOI: 10.1038/nature17970
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/nature17970
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/nature17970?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Hong-Wei Zhang & Kun Huang & Zhan-Xi Gu & Xiao-Xian Wu & Jia-Wei Wang & Yu Zhang, 2023. "A cryo-EM structure of KTF1-bound polymerase V transcription elongation complex," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    2. Simona Pilotto & Michal Sýkora & Gwenny Cackett & Christopher Dulson & Finn Werner, 2024. "Structure of the recombinant RNA polymerase from African Swine Fever Virus," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    3. Benjamin M. Spector & Mrutyunjaya Parida & Ming Li & Christopher B. Ball & Jeffery L. Meier & Donal S. Luse & David H. Price, 2022. "Differences in RNA polymerase II complexes and their interactions with surrounding chromatin on human and cytomegalovirus genomes," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
    4. Jina Yu & Chunli Yan & Thomas Dodd & Chi-Lin Tsai & John A. Tainer & Susan E. Tsutakawa & Ivaylo Ivanov, 2023. "Dynamic conformational switching underlies TFIIH function in transcription and DNA repair and impacts genetic diseases," Nature Communications, Nature, vol. 14(1), pages 1-15, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:533:y:2016:i:7603:d:10.1038_nature17970. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.