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The crystal structure of Cpf1 in complex with CRISPR RNA

Author

Listed:
  • De Dong

    (School of Life Science and Technology, Harbin Institute of Technology)

  • Kuan Ren

    (School of Life Science and Technology, Harbin Institute of Technology)

  • Xiaolin Qiu

    (School of Life Science and Technology, Harbin Institute of Technology)

  • Jianlin Zheng

    (School of Life Science and Technology, Harbin Institute of Technology)

  • Minghui Guo

    (School of Life Science and Technology, Harbin Institute of Technology)

  • Xiaoyu Guan

    (School of Life Science and Technology, Harbin Institute of Technology)

  • Hongnan Liu

    (School of Life Science and Technology, Harbin Institute of Technology)

  • Ningning Li

    (Ministry of Education Key Laboratory of Protein Sciences, Center for Structural Biology, School of Life Sciences, Tsinghua University)

  • Bailing Zhang

    (School of Life Science and Technology, Harbin Institute of Technology)

  • Daijun Yang

    (School of Life Science and Technology, Harbin Institute of Technology)

  • Chuang Ma

    (School of Life Science and Technology, Harbin Institute of Technology)

  • Shuo Wang

    (School of Life Science and Technology, Harbin Institute of Technology)

  • Dan Wu

    (School of Life Science and Technology, Harbin Institute of Technology)

  • Yunfeng Ma

    (School of Life Science and Technology, Harbin Institute of Technology)

  • Shilong Fan

    (Ministry of Education Key Laboratory of Protein Sciences, Center for Structural Biology, School of Life Sciences, Tsinghua University)

  • Jiawei Wang

    (Ministry of Education Key Laboratory of Protein Sciences, Center for Structural Biology, School of Life Sciences, Tsinghua University)

  • Ning Gao

    (Ministry of Education Key Laboratory of Protein Sciences, Center for Structural Biology, School of Life Sciences, Tsinghua University)

  • Zhiwei Huang

    (School of Life Science and Technology, Harbin Institute of Technology)

Abstract

The crystal structure of monomeric Lachnospiraceae bacterium Cpf1 protein bound to CRISPR RNA is presented, establishing a framework for engineering LbCpf1 to improve its efficiency and specificity for genome editing.

Suggested Citation

  • De Dong & Kuan Ren & Xiaolin Qiu & Jianlin Zheng & Minghui Guo & Xiaoyu Guan & Hongnan Liu & Ningning Li & Bailing Zhang & Daijun Yang & Chuang Ma & Shuo Wang & Dan Wu & Yunfeng Ma & Shilong Fan & Jia, 2016. "The crystal structure of Cpf1 in complex with CRISPR RNA," Nature, Nature, vol. 532(7600), pages 522-526, April.
    • Handle: RePEc:nat:nature:v:532:y:2016:i:7600:d:10.1038_nature17944
    DOI: 10.1038/nature17944
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    Cited by:

    1. Aakash Saha & Mohd Ahsan & Pablo R. Arantes & Michael Schmitz & Christelle Chanez & Martin Jinek & Giulia Palermo, 2024. "An alpha-helical lid guides the target DNA toward catalysis in CRISPR-Cas12a," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    2. Eman A. Ageely & Ramadevi Chilamkurthy & Sunit Jana & Leonora Abdullahu & Daniel O’Reilly & Philip J. Jensik & Masad J. Damha & Keith T. Gagnon, 2021. "Gene editing with CRISPR-Cas12a guides possessing ribose-modified pseudoknot handles," Nature Communications, Nature, vol. 12(1), pages 1-15, December.

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