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Crystal structures of a polypeptide processing and secretion transporter

Author

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  • David Yin-wei Lin

    (Laboratory of Membrane Biology and Biophysics, The Rockefeller University
    Howard Hughes Medical Institute)

  • Shuo Huang

    (Howard Hughes Medical Institute
    †Present address: School of Chemistry and Biochemistry, Georgia Institute of Technology, 901 Atlantic Drive, Atlanta, Georgia 30332, USA.)

  • Jue Chen

    (Laboratory of Membrane Biology and Biophysics, The Rockefeller University
    Howard Hughes Medical Institute)

Abstract

Bacteria secrete peptides and proteins to communicate, to poison competitors, and to manipulate host cells. Among the various protein-translocation machineries, the peptidase-containing ATP-binding cassette transporters (PCATs) are appealingly simple. Each PCAT contains two peptidase domains that cleave the secretion signal from the substrate, two transmembrane domains that form a translocation pathway, and two nucleotide-binding domains that hydrolyse ATP. In Gram-positive bacteria, PCATs function both as maturation proteases and exporters for quorum-sensing or antimicrobial polypeptides. In Gram-negative bacteria, PCATs interact with two other membrane proteins to form the type 1 secretion system. Here we present crystal structures of PCAT1 from Clostridium thermocellum in two different conformations. These structures, accompanied by biochemical data, show that the translocation pathway is a large α-helical barrel sufficient to accommodate small folded proteins. ATP binding alternates access to the transmembrane pathway and also regulates the protease activity, thereby coupling substrate processing to translocation.

Suggested Citation

  • David Yin-wei Lin & Shuo Huang & Jue Chen, 2015. "Crystal structures of a polypeptide processing and secretion transporter," Nature, Nature, vol. 523(7561), pages 425-430, July.
  • Handle: RePEc:nat:nature:v:523:y:2015:i:7561:d:10.1038_nature14623
    DOI: 10.1038/nature14623
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    Cited by:

    1. Lin Yu & Xin Xu & Wan-Zhen Chua & Hao Feng & Zheng Ser & Kai Shao & Jian Shi & Yumei Wang & Zongli Li & Radoslaw M. Sobota & Lok-To Sham & Min Luo, 2023. "Structural basis of peptide secretion for Quorum sensing by ComA," Nature Communications, Nature, vol. 14(1), pages 1-17, December.

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