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An alternative pluripotent state confers interspecies chimaeric competency

Author

Listed:
  • Jun Wu

    (The Salk Institute for Biological Studies, Gene Expression Laboratory)

  • Daiji Okamura

    (The Salk Institute for Biological Studies, Gene Expression Laboratory
    †Present address: Department of Advanced Bioscience, Graduate School of Agriculture, Kinki University, 3327-204 Nakamachi, Nara 631-8505, Japan.)

  • Mo Li

    (The Salk Institute for Biological Studies, Gene Expression Laboratory)

  • Keiichiro Suzuki

    (The Salk Institute for Biological Studies, Gene Expression Laboratory)

  • Chongyuan Luo

    (Howard Hughes Medical Institute, The Salk Institute for Biological Studies
    The Salk Institute for Biological Studies, Genomic Analysis Laboratory)

  • Li Ma

    (The Salk Institute for Biological Studies, Gene Expression Laboratory)

  • Yupeng He

    (The Salk Institute for Biological Studies, Genomic Analysis Laboratory)

  • Zhongwei Li

    (The Salk Institute for Biological Studies, Gene Expression Laboratory)

  • Chris Benner

    (The Salk Institute for Biological Studies, Integrated Genomics)

  • Isao Tamura

    (The Salk Institute for Biological Studies, Gene Expression Laboratory)

  • Marie N. Krause

    (The Salk Institute for Biological Studies, Gene Expression Laboratory)

  • Joseph R. Nery

    (The Salk Institute for Biological Studies, Genomic Analysis Laboratory)

  • Tingting Du

    (Ludwig Institute for Cancer Research, University of California, San Diego School of Medicine)

  • Zhuzhu Zhang

    (The Salk Institute for Biological Studies, Genomic Analysis Laboratory)

  • Tomoaki Hishida

    (The Salk Institute for Biological Studies, Gene Expression Laboratory)

  • Yuta Takahashi

    (The Salk Institute for Biological Studies, Gene Expression Laboratory
    Life Science Center, Tsukuba Advanced Research Alliance, University of Tsukuba)

  • Emi Aizawa

    (The Salk Institute for Biological Studies, Gene Expression Laboratory)

  • Na Young Kim

    (The Salk Institute for Biological Studies, Gene Expression Laboratory)

  • Jeronimo Lajara

    (Grado en Medicina, Universidad Católica, San Antonio de Murcia)

  • Pedro Guillen

    (Grado en Medicina, Universidad Católica, San Antonio de Murcia
    Fundacion Pedro Guillen, Clínica Cemtro)

  • Josep M. Campistol

    (Hospital Clinic of Barcelona)

  • Concepcion Rodriguez Esteban

    (The Salk Institute for Biological Studies, Gene Expression Laboratory)

  • Pablo J. Ross

    (University of California, Davis)

  • Alan Saghatelian

    (The Salk Institute for Biological Studies, Peptide Biology Laboratory)

  • Bing Ren

    (Ludwig Institute for Cancer Research, University of California, San Diego School of Medicine)

  • Joseph R. Ecker

    (Howard Hughes Medical Institute, The Salk Institute for Biological Studies
    The Salk Institute for Biological Studies, Genomic Analysis Laboratory)

  • Juan Carlos Izpisua Belmonte

    (The Salk Institute for Biological Studies, Gene Expression Laboratory)

Abstract

Pluripotency, the ability to generate any cell type of the body, is an evanescent attribute of embryonic cells. Transitory pluripotent cells can be captured at different time points during embryogenesis and maintained as embryonic stem cells or epiblast stem cells in culture. Since ontogenesis is a dynamic process in both space and time, it seems counterintuitive that these two temporal states represent the full spectrum of organismal pluripotency. Here we show that by modulating culture parameters, a stem-cell type with unique spatial characteristics and distinct molecular and functional features, designated as region-selective pluripotent stem cells (rsPSCs), can be efficiently obtained from mouse embryos and primate pluripotent stem cells, including humans. The ease of culturing and editing the genome of human rsPSCs offers advantages for regenerative medicine applications. The unique ability of human rsPSCs to generate post-implantation interspecies chimaeric embryos may facilitate our understanding of early human development and evolution.

Suggested Citation

  • Jun Wu & Daiji Okamura & Mo Li & Keiichiro Suzuki & Chongyuan Luo & Li Ma & Yupeng He & Zhongwei Li & Chris Benner & Isao Tamura & Marie N. Krause & Joseph R. Nery & Tingting Du & Zhuzhu Zhang & Tomoa, 2015. "An alternative pluripotent state confers interspecies chimaeric competency," Nature, Nature, vol. 521(7552), pages 316-321, May.
    • Handle: RePEc:nat:nature:v:521:y:2015:i:7552:d:10.1038_nature14413
    DOI: 10.1038/nature14413
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