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Reductive dehalogenase structure suggests a mechanism for B12-dependent dehalogenation

Author

Listed:
  • Karl A. P. Payne

    (Manchester Institute for Biotechnology, University of Manchester, 131 Princess Street, Manchester M1 7DN, UK)

  • Carolina P. Quezada

    (Manchester Institute for Biotechnology, University of Manchester, 131 Princess Street, Manchester M1 7DN, UK)

  • Karl Fisher

    (Manchester Institute for Biotechnology, University of Manchester, 131 Princess Street, Manchester M1 7DN, UK)

  • Mark S. Dunstan

    (Manchester Institute for Biotechnology, University of Manchester, 131 Princess Street, Manchester M1 7DN, UK)

  • Fraser A. Collins

    (Manchester Institute for Biotechnology, University of Manchester, 131 Princess Street, Manchester M1 7DN, UK)

  • Hanno Sjuts

    (Manchester Institute for Biotechnology, University of Manchester, 131 Princess Street, Manchester M1 7DN, UK
    Present address: Institute of Biochemistry, Goethe Universität Frankfurt, Max-von-Laue-Straße 9, 60438 Frankfurt, Germany.)

  • Colin Levy

    (Manchester Institute for Biotechnology, University of Manchester, 131 Princess Street, Manchester M1 7DN, UK)

  • Sam Hay

    (Manchester Institute for Biotechnology, University of Manchester, 131 Princess Street, Manchester M1 7DN, UK)

  • Stephen E. J. Rigby

    (Manchester Institute for Biotechnology, University of Manchester, 131 Princess Street, Manchester M1 7DN, UK)

  • David Leys

    (Manchester Institute for Biotechnology, University of Manchester, 131 Princess Street, Manchester M1 7DN, UK)

Abstract

X-ray crystallography and EPR spectroscopy are used to characterize a soluble, oxygen-tolerant reductive dehalogenase from Nitratireductor pacificus pht-3B; the data suggest that the cobalt in the cobalamin cofactor ligates the halogen atom of the substrate, directly abstracting the halogen atom via an oxidative addition.

Suggested Citation

  • Karl A. P. Payne & Carolina P. Quezada & Karl Fisher & Mark S. Dunstan & Fraser A. Collins & Hanno Sjuts & Colin Levy & Sam Hay & Stephen E. J. Rigby & David Leys, 2015. "Reductive dehalogenase structure suggests a mechanism for B12-dependent dehalogenation," Nature, Nature, vol. 517(7535), pages 513-516, January.
    • Handle: RePEc:nat:nature:v:517:y:2015:i:7535:d:10.1038_nature13901
    DOI: 10.1038/nature13901
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