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In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system

Author

Listed:
  • Danilo Maddalo

    (Memorial Sloan Kettering Cancer Center, Cancer Biology and Genetics Program, 1275 York Avenue, New York, New York 10065, USA)

  • Eusebio Manchado

    (Memorial Sloan Kettering Cancer Center, Cancer Biology and Genetics Program, 1275 York Avenue, New York, New York 10065, USA)

  • Carla P. Concepcion

    (Memorial Sloan Kettering Cancer Center, Cancer Biology and Genetics Program, 1275 York Avenue, New York, New York 10065, USA
    Weill Cornell Graduate School of Medical Sciences of Cornell University, 1300 York Avenue)

  • Ciro Bonetti

    (Memorial Sloan Kettering Cancer Center, Cancer Biology and Genetics Program, 1275 York Avenue, New York, New York 10065, USA)

  • Joana A. Vidigal

    (Memorial Sloan Kettering Cancer Center, Cancer Biology and Genetics Program, 1275 York Avenue, New York, New York 10065, USA)

  • Yoon-Chi Han

    (Memorial Sloan Kettering Cancer Center, Cancer Biology and Genetics Program, 1275 York Avenue, New York, New York 10065, USA)

  • Paul Ogrodowski

    (Memorial Sloan Kettering Cancer Center, Cancer Biology and Genetics Program, 1275 York Avenue, New York, New York 10065, USA)

  • Alessandra Crippa

    (Milano-Bicocca University, San Gerardo Hospital, 20052, Via G B Pergolesi 33, Monza, Italy)

  • Natasha Rekhtman

    (Memorial Sloan Kettering Cancer Center, Thoracic Pathology and Cytopathology, 1275 York Avenue, New York, New York 10065, USA)

  • Elisa de Stanchina

    (Memorial Sloan Kettering Cancer Center, Molecular Pharmacology Program, 1275 York Avenue, New York, New York 10065, USA)

  • Scott W. Lowe

    (Memorial Sloan Kettering Cancer Center, Cancer Biology and Genetics Program, 1275 York Avenue, New York, New York 10065, USA
    Howard Hughes Medical Institute, 1275 York Avenue)

  • Andrea Ventura

    (Memorial Sloan Kettering Cancer Center, Cancer Biology and Genetics Program, 1275 York Avenue, New York, New York 10065, USA)

Abstract

The CRISPR/Cas system has been used to induce the Eml4–Alk chromosomal inversion in mice, a characteristic chromosomal rearrangement seen in human non-small cell lung cancers; the mice developed lung cancer and responded to the ALK inhibitor crizotinib, which is used to treat lung cancer patients with the EML4–ALK rearrangement; this general strategy can be used to engineer other disease-associated chromosomal rearrangements in mice and potentially in other organisms.

Suggested Citation

  • Danilo Maddalo & Eusebio Manchado & Carla P. Concepcion & Ciro Bonetti & Joana A. Vidigal & Yoon-Chi Han & Paul Ogrodowski & Alessandra Crippa & Natasha Rekhtman & Elisa de Stanchina & Scott W. Lowe &, 2014. "In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system," Nature, Nature, vol. 516(7531), pages 423-427, December.
  • Handle: RePEc:nat:nature:v:516:y:2014:i:7531:d:10.1038_nature13902
    DOI: 10.1038/nature13902
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    Cited by:

    1. Yuanyuan Chen & Reka Toth & Sara Chocarro & Dieter Weichenhan & Joschka Hey & Pavlo Lutsik & Stefan Sawall & Georgios T. Stathopoulos & Christoph Plass & Rocio Sotillo, 2022. "Club cells employ regeneration mechanisms during lung tumorigenesis," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    2. Ava P. Amini & Jesse D. Kirkpatrick & Cathy S. Wang & Alex M. Jaeger & Susan Su & Santiago Naranjo & Qian Zhong & Christina M. Cabana & Tyler Jacks & Sangeeta N. Bhatia, 2022. "Multiscale profiling of protease activity in cancer," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    3. Ryan J. Geusz & Allen Wang & Dieter K. Lam & Nicholas K. Vinckier & Konstantinos-Dionysios Alysandratos & David A. Roberts & Jinzhao Wang & Samy Kefalopoulou & Araceli Ramirez & Yunjiang Qiu & Joshua , 2021. "Sequence logic at enhancers governs a dual mechanism of endodermal organ fate induction by FOXA pioneer factors," Nature Communications, Nature, vol. 12(1), pages 1-19, December.
    4. Charlotte Douglas & Valdone Maciulyte & Jasmin Zohren & Daniel M. Snell & Shantha K. Mahadevaiah & Obah A. Ojarikre & Peter J. I. Ellis & James M. A. Turner, 2021. "CRISPR-Cas9 effectors facilitate generation of single-sex litters and sex-specific phenotypes," Nature Communications, Nature, vol. 12(1), pages 1-10, December.
    5. Taek-Chin Cheong & Ahram Jang & Qi Wang & Giulia C. Leonardi & Biagio Ricciuti & Joao V. Alessi & Alessandro Di Federico & Mark M. Awad & Maria K. Lehtinen & Marian H. Harris & Roberto Chiarle, 2024. "Mechanistic patterns and clinical implications of oncogenic tyrosine kinase fusions in human cancers," Nature Communications, Nature, vol. 15(1), pages 1-19, December.

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