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Multiplex single-molecule interaction profiling of DNA-barcoded proteins

Author

Listed:
  • Liangcai Gu

    (Harvard Medical School)

  • Chao Li

    (Wyss Institute for Biologically Inspired Engineering, Harvard University)

  • John Aach

    (Harvard Medical School)

  • David E. Hill

    (Harvard Medical School
    Dana-Farber Cancer Institute)

  • Marc Vidal

    (Harvard Medical School
    Dana-Farber Cancer Institute)

  • George M. Church

    (Harvard Medical School
    Wyss Institute for Biologically Inspired Engineering, Harvard University)

Abstract

Single-molecular-interaction-sequencing involves attaching DNA barcodes to proteins, assaying these barcoded proteins en masse in an aqueous solution, followed by immobilization in a polyacrylamide film and amplifying and analysing the barcoding DNAs—the method allows for precise protein quantification and simultaneous interrogation of molecular binding affinity and specificity.

Suggested Citation

  • Liangcai Gu & Chao Li & John Aach & David E. Hill & Marc Vidal & George M. Church, 2014. "Multiplex single-molecule interaction profiling of DNA-barcoded proteins," Nature, Nature, vol. 515(7528), pages 554-557, November.
  • Handle: RePEc:nat:nature:v:515:y:2014:i:7528:d:10.1038_nature13761
    DOI: 10.1038/nature13761
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