IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v514y2014i7522d10.1038_nature13589.html
   My bibliography  Save this article

CRISPR-mediated direct mutation of cancer genes in the mouse liver

Author

Listed:
  • Wen Xue

    (David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology)

  • Sidi Chen

    (David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology)

  • Hao Yin

    (David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology)

  • Tuomas Tammela

    (David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology)

  • Thales Papagiannakopoulos

    (David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology)

  • Nikhil S. Joshi

    (David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology)

  • Wenxin Cai

    (David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology)

  • Gillian Yang

    (David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology)

  • Roderick Bronson

    (Tufts University and Harvard Medical School)

  • Denise G. Crowley

    (David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology)

  • Feng Zhang

    (Broad Institute of Massachusetts Institute of Technology and Harvard)

  • Daniel G. Anderson

    (David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology
    Massachusetts Institute of Technology
    Harvard-MIT Division of Health Sciences & Technology
    Institute for Medical Engineering and Science, Massachusetts Institute of Technology)

  • Phillip A. Sharp

    (David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology
    Massachusetts Institute of Technology)

  • Tyler Jacks

    (David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology
    Massachusetts Institute of Technology
    Howard Hughes Medical Institute, Massachusetts Institute of Technology)

Abstract

CRISPR plasmids targeting Pten and p53, alone and in combination, are delivered by hydrodynamic injection to the liver; the CRISPR-mediated mutations phenocopy the effects of deletions using Cre–LoxP technology, allowing the direct mutation of tumour suppressor genes and oncogenes in the liver using the CRISPR/Cas system, which presents a new approach for rapid development of liver cancer models and functional genomics.

Suggested Citation

  • Wen Xue & Sidi Chen & Hao Yin & Tuomas Tammela & Thales Papagiannakopoulos & Nikhil S. Joshi & Wenxin Cai & Gillian Yang & Roderick Bronson & Denise G. Crowley & Feng Zhang & Daniel G. Anderson & Phil, 2014. "CRISPR-mediated direct mutation of cancer genes in the mouse liver," Nature, Nature, vol. 514(7522), pages 380-384, October.
    • Handle: RePEc:nat:nature:v:514:y:2014:i:7522:d:10.1038_nature13589
    DOI: 10.1038/nature13589
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/nature13589
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/nature13589?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Christel F. A. Ramirez & Daniel Taranto & Masami Ando-Kuri & Marnix H. P. Groot & Efi Tsouri & Zhijie Huang & Daniel Groot & Roelof J. C. Kluin & Daan J. Kloosterman & Joanne Verheij & Jing Xu & Seren, 2024. "Cancer cell genetics shaping of the tumor microenvironment reveals myeloid cell-centric exploitable vulnerabilities in hepatocellular carcinoma," Nature Communications, Nature, vol. 15(1), pages 1-24, December.
    2. Duško Lainšček & Vida Forstnerič & Veronika Mikolič & Špela Malenšek & Peter Pečan & Mojca Benčina & Matjaž Sever & Helena Podgornik & Roman Jerala, 2022. "Coiled-coil heterodimer-based recruitment of an exonuclease to CRISPR/Cas for enhanced gene editing," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    3. Yazhong Cui & Yang Miao & Longzhi Cao & Lifang Guo & Yue Cui & Chuanzhe Yan & Zhi Zeng & Mo Xu & Ting Han, 2023. "Activation of melanocortin-1 receptor signaling in melanoma cells impairs T cell infiltration to dampen antitumor immunity," Nature Communications, Nature, vol. 14(1), pages 1-18, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:514:y:2014:i:7522:d:10.1038_nature13589. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.