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Saturation editing of genomic regions by multiplex homology-directed repair

Author

Listed:
  • Gregory M. Findlay

    (University of Washington)

  • Evan A. Boyle

    (University of Washington)

  • Ronald J. Hause

    (University of Washington)

  • Jason C. Klein

    (University of Washington)

  • Jay Shendure

    (University of Washington)

Abstract

The authors perform saturation mutagenesis of genomic regions in their native endogenous chromosomal context by using CRISPR/Cas9 RNA-guided cleavage and multiplex homology-directed repair; its utility is demonstrated by measuring the effects of hundreds to thousands of genomic edits to BRCA1 and DBR1 on splicing and cellular fitness, respectively.

Suggested Citation

  • Gregory M. Findlay & Evan A. Boyle & Ronald J. Hause & Jason C. Klein & Jay Shendure, 2014. "Saturation editing of genomic regions by multiplex homology-directed repair," Nature, Nature, vol. 513(7516), pages 120-123, September.
    • Handle: RePEc:nat:nature:v:513:y:2014:i:7516:d:10.1038_nature13695
    DOI: 10.1038/nature13695
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    Cited by:

    1. Luke Buerer & Nathaniel E. Clark & Anastasia Welch & Chaorui Duan & Allison J. Taggart & Brittany A. Townley & Jing Wang & Rachel Soemedi & Stephen Rong & Chien-Ling Lin & Yi Zeng & Adam Katolik & Jon, 2024. "The debranching enzyme Dbr1 regulates lariat turnover and intron splicing," Nature Communications, Nature, vol. 15(1), pages 1-13, December.

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