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A draft map of the human proteome

Author

Listed:
  • Min-Sik Kim

    (McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine
    Johns Hopkins University School of Medicine)

  • Sneha M. Pinto

    (Institute of Bioinformatics, International Tech Park, Bangalore 560066, India)

  • Derese Getnet

    (McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine
    Adrienne Helis Malvin Medical Research Foundation)

  • Raja Sekhar Nirujogi

    (Institute of Bioinformatics, International Tech Park, Bangalore 560066, India)

  • Srikanth S. Manda

    (Institute of Bioinformatics, International Tech Park, Bangalore 560066, India)

  • Raghothama Chaerkady

    (McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine
    Johns Hopkins University School of Medicine)

  • Anil K. Madugundu

    (Institute of Bioinformatics, International Tech Park, Bangalore 560066, India)

  • Dhanashree S. Kelkar

    (Institute of Bioinformatics, International Tech Park, Bangalore 560066, India)

  • Ruth Isserlin

    (The Donnelly Centre, University of Toronto, Toronto, Ontario M5S 3E1, Canada)

  • Shobhit Jain

    (The Donnelly Centre, University of Toronto, Toronto, Ontario M5S 3E1, Canada)

  • Joji K. Thomas

    (Institute of Bioinformatics, International Tech Park, Bangalore 560066, India)

  • Babylakshmi Muthusamy

    (Institute of Bioinformatics, International Tech Park, Bangalore 560066, India)

  • Pamela Leal-Rojas

    (McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine
    Universidad de La Frontera, Center of Genetic and Immunological Studies-Scientific and Technological Bioresource Nucleus, Temuco 4811230, Chile)

  • Praveen Kumar

    (Institute of Bioinformatics, International Tech Park, Bangalore 560066, India)

  • Nandini A. Sahasrabuddhe

    (Institute of Bioinformatics, International Tech Park, Bangalore 560066, India)

  • Lavanya Balakrishnan

    (Institute of Bioinformatics, International Tech Park, Bangalore 560066, India)

  • Jayshree Advani

    (Institute of Bioinformatics, International Tech Park, Bangalore 560066, India)

  • Bijesh George

    (Institute of Bioinformatics, International Tech Park, Bangalore 560066, India)

  • Santosh Renuse

    (Institute of Bioinformatics, International Tech Park, Bangalore 560066, India)

  • Lakshmi Dhevi N. Selvan

    (Institute of Bioinformatics, International Tech Park, Bangalore 560066, India)

  • Arun H. Patil

    (Institute of Bioinformatics, International Tech Park, Bangalore 560066, India)

  • Vishalakshi Nanjappa

    (Institute of Bioinformatics, International Tech Park, Bangalore 560066, India)

  • Aneesha Radhakrishnan

    (Institute of Bioinformatics, International Tech Park, Bangalore 560066, India)

  • Samarjeet Prasad

    (McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine)

  • Tejaswini Subbannayya

    (Institute of Bioinformatics, International Tech Park, Bangalore 560066, India)

  • Rajesh Raju

    (Institute of Bioinformatics, International Tech Park, Bangalore 560066, India)

  • Manish Kumar

    (Institute of Bioinformatics, International Tech Park, Bangalore 560066, India)

  • Sreelakshmi K. Sreenivasamurthy

    (Institute of Bioinformatics, International Tech Park, Bangalore 560066, India)

  • Arivusudar Marimuthu

    (Institute of Bioinformatics, International Tech Park, Bangalore 560066, India)

  • Gajanan J. Sathe

    (Institute of Bioinformatics, International Tech Park, Bangalore 560066, India)

  • Sandip Chavan

    (Institute of Bioinformatics, International Tech Park, Bangalore 560066, India)

  • Keshava K. Datta

    (Institute of Bioinformatics, International Tech Park, Bangalore 560066, India)

  • Yashwanth Subbannayya

    (Institute of Bioinformatics, International Tech Park, Bangalore 560066, India)

  • Apeksha Sahu

    (Institute of Bioinformatics, International Tech Park, Bangalore 560066, India)

  • Soujanya D. Yelamanchi

    (Institute of Bioinformatics, International Tech Park, Bangalore 560066, India)

  • Savita Jayaram

    (Institute of Bioinformatics, International Tech Park, Bangalore 560066, India)

  • Pavithra Rajagopalan

    (Institute of Bioinformatics, International Tech Park, Bangalore 560066, India)

  • Jyoti Sharma

    (Institute of Bioinformatics, International Tech Park, Bangalore 560066, India)

  • Krishna R. Murthy

    (Institute of Bioinformatics, International Tech Park, Bangalore 560066, India)

  • Nazia Syed

    (Institute of Bioinformatics, International Tech Park, Bangalore 560066, India)

  • Renu Goel

    (Institute of Bioinformatics, International Tech Park, Bangalore 560066, India)

  • Aafaque A. Khan

    (Institute of Bioinformatics, International Tech Park, Bangalore 560066, India)

  • Sartaj Ahmad

    (Institute of Bioinformatics, International Tech Park, Bangalore 560066, India)

  • Gourav Dey

    (Institute of Bioinformatics, International Tech Park, Bangalore 560066, India)

  • Keshav Mudgal

    (School of Medicine, Imperial College London, South Kensington Campus, London SW7 2AZ, UK)

  • Aditi Chatterjee

    (Institute of Bioinformatics, International Tech Park, Bangalore 560066, India)

  • Tai-Chung Huang

    (McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine)

  • Jun Zhong

    (McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine)

  • Xinyan Wu

    (McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine
    Johns Hopkins University School of Medicine)

  • Patrick G. Shaw

    (McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine)

  • Donald Freed

    (McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine)

  • Muhammad S. Zahari

    (Johns Hopkins University School of Medicine)

  • Kanchan K. Mukherjee

    (Postgraduate Institute of Medical Education & Research, Chandigarh 160012, India)

  • Subramanian Shankar

    (Pune 411040, India)

  • Anita Mahadevan

    (National Institute of Mental Health and Neurosciences, Bangalore 560029, India
    Human Brain Tissue Repository, Neurobiology Research Centre, National Institute of Mental Health and Neurosciences, Bangalore 560029, India)

  • Henry Lam

    (The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong)

  • Christopher J. Mitchell

    (McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine)

  • Susarla Krishna Shankar

    (National Institute of Mental Health and Neurosciences, Bangalore 560029, India
    Human Brain Tissue Repository, Neurobiology Research Centre, National Institute of Mental Health and Neurosciences, Bangalore 560029, India)

  • Parthasarathy Satishchandra

    (National Institute of Mental Health and Neurosciences, Bangalore 560029, India)

  • John T. Schroeder

    (Johns Hopkins University School of Medicine)

  • Ravi Sirdeshmukh

    (Institute of Bioinformatics, International Tech Park, Bangalore 560066, India)

  • Anirban Maitra

    (The Sol Goldman Pancreatic Cancer Research Center, Johns Hopkins University School of Medicine
    Johns Hopkins University School of Medicine)

  • Steven D. Leach

    (McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine
    Johns Hopkins University School of Medicine)

  • Charles G. Drake

    (Johns Hopkins University School of Medicine
    Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine)

  • Marc K. Halushka

    (The Sol Goldman Pancreatic Cancer Research Center, Johns Hopkins University School of Medicine)

  • T. S. Keshava Prasad

    (Institute of Bioinformatics, International Tech Park, Bangalore 560066, India)

  • Ralph H. Hruban

    (The Sol Goldman Pancreatic Cancer Research Center, Johns Hopkins University School of Medicine
    Johns Hopkins University School of Medicine)

  • Candace L. Kerr

    (Johns Hopkins University School of Medicine Baltimore
    Present address: Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA.)

  • Gary D. Bader

    (The Donnelly Centre, University of Toronto, Toronto, Ontario M5S 3E1, Canada)

  • Christine A. Iacobuzio-Donahue

    (The Sol Goldman Pancreatic Cancer Research Center, Johns Hopkins University School of Medicine
    Johns Hopkins University School of Medicine
    Johns Hopkins University School of Medicine)

  • Harsha Gowda

    (Institute of Bioinformatics, International Tech Park, Bangalore 560066, India)

  • Akhilesh Pandey

    (McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine
    Johns Hopkins University School of Medicine
    Institute of Bioinformatics, International Tech Park, Bangalore 560066, India
    Adrienne Helis Malvin Medical Research Foundation)

Abstract

The availability of human genome sequence has transformed biomedical research over the past decade. However, an equivalent map for the human proteome with direct measurements of proteins and peptides does not exist yet. Here we present a draft map of the human proteome using high-resolution Fourier-transform mass spectrometry. In-depth proteomic profiling of 30 histologically normal human samples, including 17 adult tissues, 7 fetal tissues and 6 purified primary haematopoietic cells, resulted in identification of proteins encoded by 17,294 genes accounting for approximately 84% of the total annotated protein-coding genes in humans. A unique and comprehensive strategy for proteogenomic analysis enabled us to discover a number of novel protein-coding regions, which includes translated pseudogenes, non-coding RNAs and upstream open reading frames. This large human proteome catalogue (available as an interactive web-based resource at http://www.humanproteomemap.org ) will complement available human genome and transcriptome data to accelerate biomedical research in health and disease.

Suggested Citation

  • Min-Sik Kim & Sneha M. Pinto & Derese Getnet & Raja Sekhar Nirujogi & Srikanth S. Manda & Raghothama Chaerkady & Anil K. Madugundu & Dhanashree S. Kelkar & Ruth Isserlin & Shobhit Jain & Joji K. Thoma, 2014. "A draft map of the human proteome," Nature, Nature, vol. 509(7502), pages 575-581, May.
    • Handle: RePEc:nat:nature:v:509:y:2014:i:7502:d:10.1038_nature13302
    DOI: 10.1038/nature13302
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    Citations

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    Cited by:

    1. Long Wu & Ayman Hoque & Henry Lam, 2023. "Spectroscape enables real-time query and visualization of a spectral archive in proteomics," Nature Communications, Nature, vol. 14(1), pages 1-10, December.
    2. Xiaojuan Fan & Yun Yang & Chuyun Chen & Zefeng Wang, 2022. "Pervasive translation of circular RNAs driven by short IRES-like elements," Nature Communications, Nature, vol. 13(1), pages 1-15, December.

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