IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v509y2014i7501d10.1038_nature13166.html
   My bibliography  Save this article

High-throughput screening of a CRISPR/Cas9 library for functional genomics in human cells

Author

Listed:
  • Yuexin Zhou

    (State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University)

  • Shiyou Zhu

    (State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University)

  • Changzu Cai

    (State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University)

  • Pengfei Yuan

    (State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University)

  • Chunmei Li

    (Biodynamic Optical Imaging Centre (BIOPIC), College of Engineering, Peking University)

  • Yanyi Huang

    (Biodynamic Optical Imaging Centre (BIOPIC), College of Engineering, Peking University)

  • Wensheng Wei

    (State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University)

Abstract

This study describes the construction of a focused CRISPR/Cas-based lentiviral library in human cells and a method of gene identification based on functional screening and high-throughput sequencing analysis.

Suggested Citation

  • Yuexin Zhou & Shiyou Zhu & Changzu Cai & Pengfei Yuan & Chunmei Li & Yanyi Huang & Wensheng Wei, 2014. "High-throughput screening of a CRISPR/Cas9 library for functional genomics in human cells," Nature, Nature, vol. 509(7501), pages 487-491, May.
    • Handle: RePEc:nat:nature:v:509:y:2014:i:7501:d:10.1038_nature13166
    DOI: 10.1038/nature13166
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/nature13166
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/nature13166?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Yutian Zou & Shaoquan Zheng & Xinhua Xie & Feng Ye & Xiaoqian Hu & Zhi Tian & Shu-Mei Yan & Lu Yang & Yanan Kong & Yuhui Tang & Wenwen Tian & Jindong Xie & Xinpei Deng & Yan Zeng & Zhe-Sheng Chen & Ha, 2022. "N6-methyladenosine regulated FGFR4 attenuates ferroptotic cell death in recalcitrant HER2-positive breast cancer," Nature Communications, Nature, vol. 13(1), pages 1-18, December.
    2. Dmitrii Degtev & Jack Bravo & Aikaterini Emmanouilidi & Aleksandar Zdravković & Oi Kuan Choong & Julia Liz Touza & Niklas Selfjord & Isabel Weisheit & Margherita Francescatto & Pinar Akcakaya & Michel, 2024. "Engineered PsCas9 enables therapeutic genome editing in mouse liver with lipid nanoparticles," Nature Communications, Nature, vol. 15(1), pages 1-15, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:509:y:2014:i:7501:d:10.1038_nature13166. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.