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High-throughput screening of a CRISPR/Cas9 library for functional genomics in human cells

Author

Listed:
  • Yuexin Zhou

    (State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University)

  • Shiyou Zhu

    (State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University)

  • Changzu Cai

    (State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University)

  • Pengfei Yuan

    (State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University)

  • Chunmei Li

    (Biodynamic Optical Imaging Centre (BIOPIC), College of Engineering, Peking University)

  • Yanyi Huang

    (Biodynamic Optical Imaging Centre (BIOPIC), College of Engineering, Peking University)

  • Wensheng Wei

    (State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University)

Abstract

This study describes the construction of a focused CRISPR/Cas-based lentiviral library in human cells and a method of gene identification based on functional screening and high-throughput sequencing analysis.

Suggested Citation

  • Yuexin Zhou & Shiyou Zhu & Changzu Cai & Pengfei Yuan & Chunmei Li & Yanyi Huang & Wensheng Wei, 2014. "High-throughput screening of a CRISPR/Cas9 library for functional genomics in human cells," Nature, Nature, vol. 509(7501), pages 487-491, May.
    • Handle: RePEc:nat:nature:v:509:y:2014:i:7501:d:10.1038_nature13166
    DOI: 10.1038/nature13166
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    Cited by:

    1. Yutian Zou & Shaoquan Zheng & Xinhua Xie & Feng Ye & Xiaoqian Hu & Zhi Tian & Shu-Mei Yan & Lu Yang & Yanan Kong & Yuhui Tang & Wenwen Tian & Jindong Xie & Xinpei Deng & Yan Zeng & Zhe-Sheng Chen & Ha, 2022. "N6-methyladenosine regulated FGFR4 attenuates ferroptotic cell death in recalcitrant HER2-positive breast cancer," Nature Communications, Nature, vol. 13(1), pages 1-18, December.

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