IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v509y2014i7500d10.1038_nature13314.html
   My bibliography  Save this article

A semi-synthetic organism with an expanded genetic alphabet

Author

Listed:
  • Denis A. Malyshev

    (The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA)

  • Kirandeep Dhami

    (The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA)

  • Thomas Lavergne

    (The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA)

  • Tingjian Chen

    (The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA)

  • Nan Dai

    (New England Biolabs, 240 County Road)

  • Jeremy M. Foster

    (New England Biolabs, 240 County Road)

  • Ivan R. Corrêa

    (New England Biolabs, 240 County Road)

  • Floyd E. Romesberg

    (The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA)

Abstract

Triphosphates of hydrophobic nucleotides d5SICS and dNaM are imported into Escherichia coli by an exogenous algal nucleotide triphosphate transporter and then used by an endogenous polymerase to replicate, and faithfully maintain over many generations of growth, a plasmid containing the d5SICS–dNaM unnatural base pair.

Suggested Citation

  • Denis A. Malyshev & Kirandeep Dhami & Thomas Lavergne & Tingjian Chen & Nan Dai & Jeremy M. Foster & Ivan R. Corrêa & Floyd E. Romesberg, 2014. "A semi-synthetic organism with an expanded genetic alphabet," Nature, Nature, vol. 509(7500), pages 385-388, May.
    • Handle: RePEc:nat:nature:v:509:y:2014:i:7500:d:10.1038_nature13314
    DOI: 10.1038/nature13314
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/nature13314
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/nature13314?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Hinako Kawabe & Christopher A. Thomas & Shuichi Hoshika & Myong-Jung Kim & Myong-Sang Kim & Logan Miessner & Nicholas Kaplan & Jonathan M. Craig & Jens H. Gundlach & Andrew H. Laszlo & Steven A. Benne, 2023. "Enzymatic synthesis and nanopore sequencing of 12-letter supernumerary DNA," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    2. Lei Pei & Michele Garfinkel & Markus Schmidt, 2022. "Bottlenecks and opportunities for synthetic biology biosafety standards," Nature Communications, Nature, vol. 13(1), pages 1-4, December.
    3. Bang Wang & Kevin M. Bradley & Myong-Jung Kim & Roberto Laos & Cen Chen & Dietlind L. Gerloff & Luran Manfio & Zunyi Yang & Steven A. Benner, 2024. "Enzyme-assisted high throughput sequencing of an expanded genetic alphabet at single base resolution," Nature Communications, Nature, vol. 15(1), pages 1-12, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:509:y:2014:i:7500:d:10.1038_nature13314. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.