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A semi-synthetic organism with an expanded genetic alphabet

Author

Listed:
  • Denis A. Malyshev

    (The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA)

  • Kirandeep Dhami

    (The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA)

  • Thomas Lavergne

    (The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA)

  • Tingjian Chen

    (The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA)

  • Nan Dai

    (New England Biolabs, 240 County Road)

  • Jeremy M. Foster

    (New England Biolabs, 240 County Road)

  • Ivan R. Corrêa

    (New England Biolabs, 240 County Road)

  • Floyd E. Romesberg

    (The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA)

Abstract

Triphosphates of hydrophobic nucleotides d5SICS and dNaM are imported into Escherichia coli by an exogenous algal nucleotide triphosphate transporter and then used by an endogenous polymerase to replicate, and faithfully maintain over many generations of growth, a plasmid containing the d5SICS–dNaM unnatural base pair.

Suggested Citation

  • Denis A. Malyshev & Kirandeep Dhami & Thomas Lavergne & Tingjian Chen & Nan Dai & Jeremy M. Foster & Ivan R. Corrêa & Floyd E. Romesberg, 2014. "A semi-synthetic organism with an expanded genetic alphabet," Nature, Nature, vol. 509(7500), pages 385-388, May.
    • Handle: RePEc:nat:nature:v:509:y:2014:i:7500:d:10.1038_nature13314
    DOI: 10.1038/nature13314
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    Cited by:

    1. Lei Pei & Michele Garfinkel & Markus Schmidt, 2022. "Bottlenecks and opportunities for synthetic biology biosafety standards," Nature Communications, Nature, vol. 13(1), pages 1-4, December.
    2. Hinako Kawabe & Christopher A. Thomas & Shuichi Hoshika & Myong-Jung Kim & Myong-Sang Kim & Logan Miessner & Nicholas Kaplan & Jonathan M. Craig & Jens H. Gundlach & Andrew H. Laszlo & Steven A. Benne, 2023. "Enzymatic synthesis and nanopore sequencing of 12-letter supernumerary DNA," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    3. Bang Wang & Kevin M. Bradley & Myong-Jung Kim & Roberto Laos & Cen Chen & Dietlind L. Gerloff & Luran Manfio & Zunyi Yang & Steven A. Benner, 2024. "Enzyme-assisted high throughput sequencing of an expanded genetic alphabet at single base resolution," Nature Communications, Nature, vol. 15(1), pages 1-12, December.

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