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C/EBPα poises B cells for rapid reprogramming into induced pluripotent stem cells

Author

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  • Bruno Di Stefano

    (Gene Regulation, Stem Cells and Cancer Programme, Centre for Genomic Regulation (CRG), Dr Aiguader 88, 08003 Barcelona, Spain
    Universitat Pompeu Fabra (UPF), Dr Aiguader 88, 08003 Barcelona, Spain)

  • Jose Luis Sardina

    (Gene Regulation, Stem Cells and Cancer Programme, Centre for Genomic Regulation (CRG), Dr Aiguader 88, 08003 Barcelona, Spain
    Universitat Pompeu Fabra (UPF), Dr Aiguader 88, 08003 Barcelona, Spain)

  • Chris van Oevelen

    (Gene Regulation, Stem Cells and Cancer Programme, Centre for Genomic Regulation (CRG), Dr Aiguader 88, 08003 Barcelona, Spain
    Universitat Pompeu Fabra (UPF), Dr Aiguader 88, 08003 Barcelona, Spain)

  • Samuel Collombet

    (Ecole Normale Supérieure, Institut de Biologie de l'ENS, 45 Rue d'Ulm, Paris F-75005, France
    Inserm, U1024, Paris F-75005, France
    CNRS, UMR 8197, Paris F-75005, France)

  • Eric M. Kallin

    (Gene Regulation, Stem Cells and Cancer Programme, Centre for Genomic Regulation (CRG), Dr Aiguader 88, 08003 Barcelona, Spain
    Universitat Pompeu Fabra (UPF), Dr Aiguader 88, 08003 Barcelona, Spain
    Present address: Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York 10065, USA.)

  • Guillermo P. Vicent

    (Gene Regulation, Stem Cells and Cancer Programme, Centre for Genomic Regulation (CRG), Dr Aiguader 88, 08003 Barcelona, Spain
    Universitat Pompeu Fabra (UPF), Dr Aiguader 88, 08003 Barcelona, Spain)

  • Jun Lu

    (Yale Cancer Center, Yale University School of Medicine, 333 Cedar Street, New Haven, Connecticut 06510, USA)

  • Denis Thieffry

    (Ecole Normale Supérieure, Institut de Biologie de l'ENS, 45 Rue d'Ulm, Paris F-75005, France
    Inserm, U1024, Paris F-75005, France
    CNRS, UMR 8197, Paris F-75005, France)

  • Miguel Beato

    (Gene Regulation, Stem Cells and Cancer Programme, Centre for Genomic Regulation (CRG), Dr Aiguader 88, 08003 Barcelona, Spain
    Universitat Pompeu Fabra (UPF), Dr Aiguader 88, 08003 Barcelona, Spain)

  • Thomas Graf

    (Gene Regulation, Stem Cells and Cancer Programme, Centre for Genomic Regulation (CRG), Dr Aiguader 88, 08003 Barcelona, Spain
    Universitat Pompeu Fabra (UPF), Dr Aiguader 88, 08003 Barcelona, Spain
    Institució Catalana de Recerca i Estudis Avançats (ICREA), Pg Lluis Companys 23, 08010 Barcelona, Spain)

Abstract

A pulse of C/EBPα followed by overexpression of the transcription factors Oct4, Sox2, Klf4 and Myc leads to fast and very efficient reprogramming of B cell precursors to induced pluripotent stem cells; C/EBPα facilitates transient chromatin accessibility and accelerates expression of pluripotency genes through a mechanism that involves activation of the Tet2 enzyme.

Suggested Citation

  • Bruno Di Stefano & Jose Luis Sardina & Chris van Oevelen & Samuel Collombet & Eric M. Kallin & Guillermo P. Vicent & Jun Lu & Denis Thieffry & Miguel Beato & Thomas Graf, 2014. "C/EBPα poises B cells for rapid reprogramming into induced pluripotent stem cells," Nature, Nature, vol. 506(7487), pages 235-239, February.
    • Handle: RePEc:nat:nature:v:506:y:2014:i:7487:d:10.1038_nature12885
    DOI: 10.1038/nature12885
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