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Visualizing virus assembly intermediates inside marine cyanobacteria

Author

Listed:
  • Wei Dai

    (National Center for Macromolecular Imaging, Baylor College of Medicine)

  • Caroline Fu

    (National Center for Macromolecular Imaging, Baylor College of Medicine)

  • Desislava Raytcheva

    (Massachusetts Institute of Technology
    Northeastern University)

  • John Flanagan

    (National Center for Macromolecular Imaging, Baylor College of Medicine
    Present address: FEI, 5350 Dawson Creek Drive, Hillsboro, Oregon 97124, USA.)

  • Htet A. Khant

    (National Center for Macromolecular Imaging, Baylor College of Medicine)

  • Xiangan Liu

    (National Center for Macromolecular Imaging, Baylor College of Medicine)

  • Ryan H. Rochat

    (National Center for Macromolecular Imaging, Baylor College of Medicine
    Program in Structural and Computational Biology and Molecular Biophysics, Baylor College of Medicine)

  • Cameron Haase-Pettingell

    (Massachusetts Institute of Technology)

  • Jacqueline Piret

    (Northeastern University)

  • Steve J. Ludtke

    (National Center for Macromolecular Imaging, Baylor College of Medicine
    Program in Structural and Computational Biology and Molecular Biophysics, Baylor College of Medicine)

  • Kuniaki Nagayama

    (National Institute for Physiological Sciences, National Institutes of Natural Sciences, 5-1 Higashiyama, Myodaiji, Okazaki 444-8787, Japan)

  • Michael F. Schmid

    (National Center for Macromolecular Imaging, Baylor College of Medicine
    Program in Structural and Computational Biology and Molecular Biophysics, Baylor College of Medicine)

  • Jonathan A. King

    (Massachusetts Institute of Technology)

  • Wah Chiu

    (National Center for Macromolecular Imaging, Baylor College of Medicine
    Program in Structural and Computational Biology and Molecular Biophysics, Baylor College of Medicine)

Abstract

This study reports the first application of Zernike phase contrast (ZPC) electron cryo-tomography to examine cellular processes without the need for labelling or sectioning; the technique is used to visualize the maturation of the cyanophage Syn5 inside its host cell, identifying subcellular compartments and five distinct Syn5 assembly intermediates.

Suggested Citation

  • Wei Dai & Caroline Fu & Desislava Raytcheva & John Flanagan & Htet A. Khant & Xiangan Liu & Ryan H. Rochat & Cameron Haase-Pettingell & Jacqueline Piret & Steve J. Ludtke & Kuniaki Nagayama & Michael , 2013. "Visualizing virus assembly intermediates inside marine cyanobacteria," Nature, Nature, vol. 502(7473), pages 707-710, October.
    • Handle: RePEc:nat:nature:v:502:y:2013:i:7473:d:10.1038_nature12604
    DOI: 10.1038/nature12604
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    Cited by:

    1. Lanlan Cai & Hang Liu & Wen Zhang & Shiwei Xiao & Qinglu Zeng & Shangyu Dang, 2023. "Cryo-EM structure of cyanophage P-SCSP1u offers insights into DNA gating and evolution of T7-like viruses," Nature Communications, Nature, vol. 14(1), pages 1-10, December.
    2. Diana P. Baquero & Sofia Medvedeva & Camille Martin-Gallausiaux & Nika Pende & Anna Sartori-Rupp & Stéphane Tachon & Thierry Pedron & Laurent Debarbieux & Guillaume Borrel & Simonetta Gribaldo & Mart , 2024. "Stable coexistence between an archaeal virus and the dominant methanogen of the human gut," Nature Communications, Nature, vol. 15(1), pages 1-14, December.

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