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Optical control of mammalian endogenous transcription and epigenetic states

Author

Listed:
  • Silvana Konermann

    (Broad Institute of MIT and Harvard, 7 Cambridge Center
    McGovern Institute for Brain Research, Massachusetts Institute of Technology)

  • Mark D. Brigham

    (Broad Institute of MIT and Harvard, 7 Cambridge Center
    McGovern Institute for Brain Research, Massachusetts Institute of Technology
    School of Engineering and Applied Sciences, Harvard University)

  • Alexandro E. Trevino

    (Broad Institute of MIT and Harvard, 7 Cambridge Center
    McGovern Institute for Brain Research, Massachusetts Institute of Technology)

  • Patrick D. Hsu

    (Broad Institute of MIT and Harvard, 7 Cambridge Center
    McGovern Institute for Brain Research, Massachusetts Institute of Technology
    Harvard University)

  • Matthias Heidenreich

    (Broad Institute of MIT and Harvard, 7 Cambridge Center
    McGovern Institute for Brain Research, Massachusetts Institute of Technology)

  • Le Cong

    (Broad Institute of MIT and Harvard, 7 Cambridge Center
    McGovern Institute for Brain Research, Massachusetts Institute of Technology
    Program in Biological and Biomedical Sciences, Harvard Medical School)

  • Randall J. Platt

    (Broad Institute of MIT and Harvard, 7 Cambridge Center
    McGovern Institute for Brain Research, Massachusetts Institute of Technology)

  • David A. Scott

    (Broad Institute of MIT and Harvard, 7 Cambridge Center
    McGovern Institute for Brain Research, Massachusetts Institute of Technology)

  • George M. Church

    (Broad Institute of MIT and Harvard, 7 Cambridge Center
    Harvard Medical School)

  • Feng Zhang

    (Broad Institute of MIT and Harvard, 7 Cambridge Center
    McGovern Institute for Brain Research, Massachusetts Institute of Technology)

Abstract

Here the customizable TALE DNA-binding domain was integrated with the light-sensitive cryptochrome 2 protein and its interacting partner (CIB1) from Arabidopsis thaliana, thereby creating an optogenetic two-hybrid system called light-inducible transcriptional effectors (LITEs); the LITE system establishes a novel mode of optogenetic control of endogenous transcription and epigenetic states.

Suggested Citation

  • Silvana Konermann & Mark D. Brigham & Alexandro E. Trevino & Patrick D. Hsu & Matthias Heidenreich & Le Cong & Randall J. Platt & David A. Scott & George M. Church & Feng Zhang, 2013. "Optical control of mammalian endogenous transcription and epigenetic states," Nature, Nature, vol. 500(7463), pages 472-476, August.
    • Handle: RePEc:nat:nature:v:500:y:2013:i:7463:d:10.1038_nature12466
    DOI: 10.1038/nature12466
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    Cited by:

    1. Ankush Auradkar & Annabel Guichard & Saluja Kaduwal & Marketta Sneider & Ethan Bier, 2023. "tgCRISPRi: efficient gene knock-down using truncated gRNAs and catalytically active Cas9," Nature Communications, Nature, vol. 14(1), pages 1-14, December.

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