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Biomimetic assembly and activation of [FeFe]-hydrogenases

Author

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  • G. Berggren

    (Laboratoire de Chimie et Biologie des Métaux (CEA/Université Grenoble 1/CNRS), 17 rue des Martyrs, F-38054 Grenoble Cedex 9, France
    Collège de France, 11 place Marcelin-Berthelot, F-75231 Paris Cedex 5, France
    Present address: Department of Biochemistry and Biophysics, Stockholm University, Svante Arrhenius väg 16, 106 91 Stockholm, Sweden.)

  • A. Adamska

    (Max-Planck-Institut für Chemische Energiekonversion, Stiftstrasse 34–36, 45470 Mülheim an der Ruhr, Germany)

  • C. Lambertz

    (Lehrstuhl Biochemie der Pflanzen, AG Photobiotechnologie, Ruhr Universität Bochum, Universitätsstrasse 150, 44801 Bochum, Germany)

  • T. R. Simmons

    (Laboratoire de Chimie et Biologie des Métaux (CEA/Université Grenoble 1/CNRS), 17 rue des Martyrs, F-38054 Grenoble Cedex 9, France)

  • J. Esselborn

    (Lehrstuhl Biochemie der Pflanzen, AG Photobiotechnologie, Ruhr Universität Bochum, Universitätsstrasse 150, 44801 Bochum, Germany)

  • M. Atta

    (Laboratoire de Chimie et Biologie des Métaux (CEA/Université Grenoble 1/CNRS), 17 rue des Martyrs, F-38054 Grenoble Cedex 9, France)

  • S. Gambarelli

    (Laboratoire de Chimie Inorganique et Biologique, (CEA-INAC, Université Grenoble 1, UMR-E 3), 17 rue des Martyrs, F-38054 Grenoble Cedex 9, France)

  • J.-M. Mouesca

    (Laboratoire de Chimie Inorganique et Biologique, (CEA-INAC, Université Grenoble 1, UMR-E 3), 17 rue des Martyrs, F-38054 Grenoble Cedex 9, France)

  • E. Reijerse

    (Max-Planck-Institut für Chemische Energiekonversion, Stiftstrasse 34–36, 45470 Mülheim an der Ruhr, Germany)

  • W. Lubitz

    (Max-Planck-Institut für Chemische Energiekonversion, Stiftstrasse 34–36, 45470 Mülheim an der Ruhr, Germany)

  • T. Happe

    (Lehrstuhl Biochemie der Pflanzen, AG Photobiotechnologie, Ruhr Universität Bochum, Universitätsstrasse 150, 44801 Bochum, Germany)

  • V. Artero

    (Laboratoire de Chimie et Biologie des Métaux (CEA/Université Grenoble 1/CNRS), 17 rue des Martyrs, F-38054 Grenoble Cedex 9, France)

  • M. Fontecave

    (Laboratoire de Chimie et Biologie des Métaux (CEA/Université Grenoble 1/CNRS), 17 rue des Martyrs, F-38054 Grenoble Cedex 9, France
    Collège de France, 11 place Marcelin-Berthelot, F-75231 Paris Cedex 5, France)

Abstract

Three synthetic mimics of the di-iron centre in [FeFe]-hydrogenases are loaded onto the HydF protein and then transferred to apo-HydA1; full activation of HydA1 was achieved only with the HydF hybrid protein that contained the mimic with an azadithiolate bridge, confirming the presence of this ligand in the active site of native [FeFe]-hydrogenases.

Suggested Citation

  • G. Berggren & A. Adamska & C. Lambertz & T. R. Simmons & J. Esselborn & M. Atta & S. Gambarelli & J.-M. Mouesca & E. Reijerse & W. Lubitz & T. Happe & V. Artero & M. Fontecave, 2013. "Biomimetic assembly and activation of [FeFe]-hydrogenases," Nature, Nature, vol. 499(7456), pages 66-69, July.
    • Handle: RePEc:nat:nature:v:499:y:2013:i:7456:d:10.1038_nature12239
    DOI: 10.1038/nature12239
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