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Pif1 family helicases suppress genome instability at G-quadruplex motifs

Author

Listed:
  • Katrin Paeschke

    (Princeton University
    Present addresses: Department of Biochemistry, Theodor Boveri-Institute, University of Würzburg, Am Hubland, D-97074 Würzburg, Germany (K.P.); Institute of Molecular Cancer Research, University of Zurich, Winterthurerstrasse 190, Zurich 8057, Switzerland (P.C.).)

  • Matthew L. Bochman

    (Princeton University)

  • P. Daniela Garcia

    (Princeton University)

  • Petr Cejka

    (and Molecular and Cellular Biology, University of California
    Present addresses: Department of Biochemistry, Theodor Boveri-Institute, University of Würzburg, Am Hubland, D-97074 Würzburg, Germany (K.P.); Institute of Molecular Cancer Research, University of Zurich, Winterthurerstrasse 190, Zurich 8057, Switzerland (P.C.).)

  • Katherine L. Friedman

    (Vanderbilt University)

  • Stephen C. Kowalczykowski

    (and Molecular and Cellular Biology, University of California)

  • Virginia A. Zakian

    (Princeton University)

Abstract

The Saccharomyces cerevisiae Pif1 helicase is the prototypical member of the Pif1 DNA helicase family, which is conserved from bacteria to humans. Here we show that exceptionally potent G-quadruplex unwinding is conserved among Pif1 helicases. Moreover, Pif1 helicases from organisms separated by more than 3 billion years of evolution suppressed DNA damage at G-quadruplex motifs in yeast. The G-quadruplex-induced damage generated in the absence of Pif1 helicases led to new genetic and epigenetic changes. Furthermore, when expressed in yeast, human PIF1 suppressed both G-quadruplex-associated DNA damage and telomere lengthening.

Suggested Citation

  • Katrin Paeschke & Matthew L. Bochman & P. Daniela Garcia & Petr Cejka & Katherine L. Friedman & Stephen C. Kowalczykowski & Virginia A. Zakian, 2013. "Pif1 family helicases suppress genome instability at G-quadruplex motifs," Nature, Nature, vol. 497(7450), pages 458-462, May.
    • Handle: RePEc:nat:nature:v:497:y:2013:i:7450:d:10.1038_nature12149
    DOI: 10.1038/nature12149
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    Cited by:

    1. Zebin Hong & Alicia K. Byrd & Jun Gao & Poulomi Das & Vanessa Qianmin Tan & Emory G. Malone & Bertha Osei & John C. Marecki & Reine U. Protacio & Wayne P. Wahls & Kevin D. Raney & Haiwei Song, 2024. "Eukaryotic Pif1 helicase unwinds G-quadruplex and dsDNA using a conserved wedge," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    2. Anna C. Papageorgiou & Michaela Pospisilova & Jakub Cibulka & Raghib Ashraf & Christopher A. Waudby & Pavel Kadeřávek & Volha Maroz & Karel Kubicek & Zbynek Prokop & Lumir Krejci & Konstantinos Tripsi, 2023. "Recognition and coacervation of G-quadruplexes by a multifunctional disordered region in RECQ4 helicase," Nature Communications, Nature, vol. 14(1), pages 1-19, December.

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