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X-ray phase-contrast in vivo microtomography probes new aspects of Xenopus gastrulation

Author

Listed:
  • Julian Moosmann

    (Institute for Photon Science and Synchrotron Radiation, Karlsruhe Institute of Technology, Hermann-von-Helmholtz-Platz 1, D-76344 Eggenstein-Leopoldshafen, Germany)

  • Alexey Ershov

    (Institute for Photon Science and Synchrotron Radiation, Karlsruhe Institute of Technology, Hermann-von-Helmholtz-Platz 1, D-76344 Eggenstein-Leopoldshafen, Germany
    Institute of Physics and Technology, National Research Tomsk Polytechnic University, Lenin Avenue 30, 634050 Tomsk, Russia)

  • Venera Altapova

    (Laboratory for Applications of Synchrotron Radiation, Karlsruhe Institute of Technology, Postfach 6980, D-76128 Karlsruhe, Germany)

  • Tilo Baumbach

    (Institute for Photon Science and Synchrotron Radiation, Karlsruhe Institute of Technology, Hermann-von-Helmholtz-Platz 1, D-76344 Eggenstein-Leopoldshafen, Germany
    Laboratory for Applications of Synchrotron Radiation, Karlsruhe Institute of Technology, Postfach 6980, D-76128 Karlsruhe, Germany)

  • Maneeshi S. Prasad

    (Northwestern University, 2205 Tech Drive, Hogan 2-100, Evanston, Illinois 60208-1230, USA)

  • Carole LaBonne

    (Northwestern University, 2205 Tech Drive, Hogan 2-100, Evanston, Illinois 60208-1230, USA)

  • Xianghui Xiao

    (Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Argonne, Illinois 60439, USA)

  • Jubin Kashef

    (Zoological Institute II, Karlsruhe Institute of Technology, Cell and Developmental Biology, Kaiserstrasse 12, D-76131 Karlsruhe, Germany)

  • Ralf Hofmann

    (Institute for Photon Science and Synchrotron Radiation, Karlsruhe Institute of Technology, Hermann-von-Helmholtz-Platz 1, D-76344 Eggenstein-Leopoldshafen, Germany)

Abstract

Opaque tissues provide a challenge for live imaging of Xenopus laevis development; a problem solved by in vivo time-lapse X-ray microtomography that is shown to provide a high-resolution three-dimensional view of structural changes and dynamics of gastrulation, and that is applied to identify and analyse new aspects of gastrulation in frog embryos.

Suggested Citation

  • Julian Moosmann & Alexey Ershov & Venera Altapova & Tilo Baumbach & Maneeshi S. Prasad & Carole LaBonne & Xianghui Xiao & Jubin Kashef & Ralf Hofmann, 2013. "X-ray phase-contrast in vivo microtomography probes new aspects of Xenopus gastrulation," Nature, Nature, vol. 497(7449), pages 374-377, May.
  • Handle: RePEc:nat:nature:v:497:y:2013:i:7449:d:10.1038_nature12116
    DOI: 10.1038/nature12116
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