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Androgenetic haploid embryonic stem cells produce live transgenic mice

Author

Listed:
  • Wei Li

    (State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
    Graduate University of Chinese Academy of Sciences)

  • Ling Shuai

    (State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
    College of Life Science, Northeast Agricultural University of China)

  • Haifeng Wan

    (State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
    Graduate University of Chinese Academy of Sciences)

  • Mingzhu Dong

    (State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
    Graduate University of Chinese Academy of Sciences)

  • Meng Wang

    (Center for Molecular Systems Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China)

  • Lisi Sang

    (State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
    Graduate University of Chinese Academy of Sciences)

  • Chunjing Feng

    (State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
    Graduate University of Chinese Academy of Sciences)

  • Guan-Zheng Luo

    (Center for Molecular Systems Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China)

  • Tianda Li

    (State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
    College of Life Science, Northeast Agricultural University of China)

  • Xin Li

    (State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
    College of Life Science, Northeast Agricultural University of China)

  • Libin Wang

    (State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
    Graduate University of Chinese Academy of Sciences)

  • Qin-Yuan Zheng

    (State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
    Graduate University of Chinese Academy of Sciences)

  • Chao Sheng

    (State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
    College of Life Science, Northeast Agricultural University of China)

  • Hua-Jun Wu

    (Graduate University of Chinese Academy of Sciences
    Center for Molecular Systems Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China)

  • Zhonghua Liu

    (College of Life Science, Northeast Agricultural University of China)

  • Lei Liu

    (State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China)

  • Liu Wang

    (State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China)

  • Xiu-Jie Wang

    (Center for Molecular Systems Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China)

  • Xiao-Yang Zhao

    (State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China)

  • Qi Zhou

    (State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China)

Abstract

Mouse androgenetic haploid embryonic stem cell lines can be established by transferring sperm into an enucleated oocyte; the cells maintain haploidy and stable growth over 30 passages, express pluripotent markers, are able to differentiate into all three germ layers, contribute to germlines of chimaeras when injected into blastocysts and can produce fertile progeny that carry genetic modifications to the next generation.

Suggested Citation

  • Wei Li & Ling Shuai & Haifeng Wan & Mingzhu Dong & Meng Wang & Lisi Sang & Chunjing Feng & Guan-Zheng Luo & Tianda Li & Xin Li & Libin Wang & Qin-Yuan Zheng & Chao Sheng & Hua-Jun Wu & Zhonghua Liu & , 2012. "Androgenetic haploid embryonic stem cells produce live transgenic mice," Nature, Nature, vol. 490(7420), pages 407-411, October.
    • Handle: RePEc:nat:nature:v:490:y:2012:i:7420:d:10.1038_nature11435
    DOI: 10.1038/nature11435
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