IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v485y2012i7399d10.1038_nature11006.html
   My bibliography  Save this article

The complex of tmRNA–SmpB and EF-G on translocating ribosomes

Author

Listed:
  • David J. F. Ramrath

    (Institut für Medizinische Physik und Biophysik, Charite – Universitätsmedizin Berlin, Ziegelstrasse 5-9, 10117 Berlin, Germany)

  • Hiroshi Yamamoto

    (Institut für Medizinische Physik und Biophysik, Charite – Universitätsmedizin Berlin, Ziegelstrasse 5-9, 10117 Berlin, Germany
    Max Planck Institute for Molecular Genetics, AG Ribosomen, Ihnestrasse 73, 14195 Berlin, Germany)

  • Kristian Rother

    (Institute of Biology and Molecular Biotechnology, Collegium Biologicum, Adam Mickiewicz University, Ulica Umultowska 89, 61-614 Poznan, Poland)

  • Daniela Wittek

    (Max Planck Institute for Molecular Genetics, AG Ribosomen, Ihnestrasse 73, 14195 Berlin, Germany)

  • Markus Pech

    (Institut für Medizinische Physik und Biophysik, Charite – Universitätsmedizin Berlin, Ziegelstrasse 5-9, 10117 Berlin, Germany
    Max Planck Institute for Molecular Genetics, AG Ribosomen, Ihnestrasse 73, 14195 Berlin, Germany)

  • Thorsten Mielke

    (Institut für Medizinische Physik und Biophysik, Charite – Universitätsmedizin Berlin, Ziegelstrasse 5-9, 10117 Berlin, Germany
    UltraStrukturNetzwerk, Max Planck Institute for Molecular Genetics, Ihnestrasse 73, 14195 Berlin, Germany)

  • Justus Loerke

    (Institut für Medizinische Physik und Biophysik, Charite – Universitätsmedizin Berlin, Ziegelstrasse 5-9, 10117 Berlin, Germany)

  • Patrick Scheerer

    (Institut für Medizinische Physik und Biophysik, Charite – Universitätsmedizin Berlin, Ziegelstrasse 5-9, 10117 Berlin, Germany)

  • Pavel Ivanov

    (Max Planck Institute for Molecular Genetics, AG Ribosomen, Ihnestrasse 73, 14195 Berlin, Germany
    M. V. Lomonosov Moscow State University, 119899 Moscow, Russia
    Present address: Brigham and Women’s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.)

  • Yoshika Teraoka

    (Max Planck Institute for Molecular Genetics, AG Ribosomen, Ihnestrasse 73, 14195 Berlin, Germany)

  • Olga Shpanchenko

    (Max Planck Institute for Molecular Genetics, AG Ribosomen, Ihnestrasse 73, 14195 Berlin, Germany
    M. V. Lomonosov Moscow State University, 119899 Moscow, Russia)

  • Knud H. Nierhaus

    (Institut für Medizinische Physik und Biophysik, Charite – Universitätsmedizin Berlin, Ziegelstrasse 5-9, 10117 Berlin, Germany
    Max Planck Institute for Molecular Genetics, AG Ribosomen, Ihnestrasse 73, 14195 Berlin, Germany)

  • Christian M. T. Spahn

    (Institut für Medizinische Physik und Biophysik, Charite – Universitätsmedizin Berlin, Ziegelstrasse 5-9, 10117 Berlin, Germany)

Abstract

Stalled bacterial ribosomes can be rescued by interaction with SmpB protein and a highly structured transfer-messenger RNA, and a cryo-electron microscopy map of this complex now shows how EF-G-dependent translocation of this non-canonical ligand is facilitated by conformational changes in the ribosome and the transfer-messenger RNA.

Suggested Citation

  • David J. F. Ramrath & Hiroshi Yamamoto & Kristian Rother & Daniela Wittek & Markus Pech & Thorsten Mielke & Justus Loerke & Patrick Scheerer & Pavel Ivanov & Yoshika Teraoka & Olga Shpanchenko & Knud , 2012. "The complex of tmRNA–SmpB and EF-G on translocating ribosomes," Nature, Nature, vol. 485(7399), pages 526-529, May.
    • Handle: RePEc:nat:nature:v:485:y:2012:i:7399:d:10.1038_nature11006
    DOI: 10.1038/nature11006
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/nature11006
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/nature11006?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:485:y:2012:i:7399:d:10.1038_nature11006. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.