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Ubiquitin-dependent regulation of COPII coat size and function

Author

Listed:
  • Lingyan Jin

    (University of California at Berkeley)

  • Kanika Bajaj Pahuja

    (University of California at Berkeley
    Howard Hughes Medical Institute, University of California at Berkeley)

  • Katherine E. Wickliffe

    (University of California at Berkeley)

  • Amita Gorur

    (University of California at Berkeley
    Howard Hughes Medical Institute, University of California at Berkeley)

  • Christine Baumgärtel

    (University of California at Berkeley)

  • Randy Schekman

    (University of California at Berkeley
    Howard Hughes Medical Institute, University of California at Berkeley)

  • Michael Rape

    (University of California at Berkeley)

Abstract

Packaging of proteins from the endoplasmic reticulum into COPII vesicles is essential for secretion. In cells, most COPII vesicles are approximately 60–80 nm in diameter, yet some must increase their size to accommodate 300–400 nm procollagen fibres or chylomicrons. Impaired COPII function results in collagen deposition defects, cranio-lenticulo-sutural dysplasia, or chylomicron retention disease, but mechanisms to enlarge COPII coats have remained elusive. Here, we identified the ubiquitin ligase CUL3–KLHL12 as a regulator of COPII coat formation. CUL3–KLHL12 catalyses the monoubiquitylation of the COPII-component SEC31 and drives the assembly of large COPII coats. As a result, ubiquitylation by CUL3–KLHL12 is essential for collagen export, yet less important for the transport of small cargo. We conclude that monoubiquitylation controls the size and function of a vesicle coat.

Suggested Citation

  • Lingyan Jin & Kanika Bajaj Pahuja & Katherine E. Wickliffe & Amita Gorur & Christine Baumgärtel & Randy Schekman & Michael Rape, 2012. "Ubiquitin-dependent regulation of COPII coat size and function," Nature, Nature, vol. 482(7386), pages 495-500, February.
    • Handle: RePEc:nat:nature:v:482:y:2012:i:7386:d:10.1038_nature10822
    DOI: 10.1038/nature10822
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    Cited by:

    1. Weize Wang & Ling Liang & Zonglin Dai & Peng Zuo & Shang Yu & Yishuo Lu & Dian Ding & Hongyi Chen & Hui Shan & Yan Jin & Youdong Mao & Yuxin Yin, 2024. "A conserved N-terminal motif of CUL3 contributes to assembly and E3 ligase activity of CRL3KLHL22," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    2. William Kasberg & Peter Luong & Kevin A. Swift & Anjon Audhya, 2023. "Nutrient deprivation alters the rate of COPII subunit recruitment at ER subdomains to tune secretory protein transport," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    3. Anthony J. Asmar & Shaun R. Abrams & Jenny Hsin & Jason C. Collins & Rita M. Yazejian & Youmei Wu & Jean Cho & Andrew D. Doyle & Samhitha Cinthala & Marleen Simon & Richard H. Jaarsveld & David B. Bec, 2023. "A ubiquitin-based effector-to-inhibitor switch coordinates early brain, craniofacial, and skin development," Nature Communications, Nature, vol. 14(1), pages 1-17, December.

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