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RNAi promotes heterochromatic silencing through replication-coupled release of RNA Pol II

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  • Mikel Zaratiegui

    (Cold Spring Harbor Laboratory, Cold Spring Harbor
    Present addresses: Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, New Jersey 08854, USA (M.Z.); Yale Stem Cell Center, Yale University, New Haven, Connecticut 06520, USA (A.K.); Creative Research Initiative Sousei, Hokkaido University, 001-0021 Sapporo, Japan (D.G.); Department of Biology, New York University, New York, New York 10003, USA (F.L.); Murdoch Children’s Research Institute, University of Melbourne, Melbourne, Victoria 3052, Australia (D.V.I.).)

  • Stephane E. Castel

    (Cold Spring Harbor Laboratory, Cold Spring Harbor
    Watson School of Biological Sciences, Cold Spring Harbor Laboratory)

  • Danielle V. Irvine

    (Cold Spring Harbor Laboratory, Cold Spring Harbor
    Present addresses: Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, New Jersey 08854, USA (M.Z.); Yale Stem Cell Center, Yale University, New Haven, Connecticut 06520, USA (A.K.); Creative Research Initiative Sousei, Hokkaido University, 001-0021 Sapporo, Japan (D.G.); Department of Biology, New York University, New York, New York 10003, USA (F.L.); Murdoch Children’s Research Institute, University of Melbourne, Melbourne, Victoria 3052, Australia (D.V.I.).)

  • Anna Kloc

    (Cold Spring Harbor Laboratory, Cold Spring Harbor
    Present addresses: Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, New Jersey 08854, USA (M.Z.); Yale Stem Cell Center, Yale University, New Haven, Connecticut 06520, USA (A.K.); Creative Research Initiative Sousei, Hokkaido University, 001-0021 Sapporo, Japan (D.G.); Department of Biology, New York University, New York, New York 10003, USA (F.L.); Murdoch Children’s Research Institute, University of Melbourne, Melbourne, Victoria 3052, Australia (D.V.I.).)

  • Jie Ren

    (Cold Spring Harbor Laboratory, Cold Spring Harbor)

  • Fei Li

    (Molecular and Cellular Biology, University of California Berkeley
    Present addresses: Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, New Jersey 08854, USA (M.Z.); Yale Stem Cell Center, Yale University, New Haven, Connecticut 06520, USA (A.K.); Creative Research Initiative Sousei, Hokkaido University, 001-0021 Sapporo, Japan (D.G.); Department of Biology, New York University, New York, New York 10003, USA (F.L.); Murdoch Children’s Research Institute, University of Melbourne, Melbourne, Victoria 3052, Australia (D.V.I.).)

  • Elisa de Castro

    (Instituto de Biología Funcional y Genómica. CSIC/Universidad de Salamanca)

  • Laura Marín

    (Instituto de Biología Funcional y Genómica. CSIC/Universidad de Salamanca)

  • An-Yun Chang

    (Cold Spring Harbor Laboratory, Cold Spring Harbor
    Molecular and Cell Biology program, Stony Brook University)

  • Derek Goto

    (Cold Spring Harbor Laboratory, Cold Spring Harbor
    Present addresses: Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, New Jersey 08854, USA (M.Z.); Yale Stem Cell Center, Yale University, New Haven, Connecticut 06520, USA (A.K.); Creative Research Initiative Sousei, Hokkaido University, 001-0021 Sapporo, Japan (D.G.); Department of Biology, New York University, New York, New York 10003, USA (F.L.); Murdoch Children’s Research Institute, University of Melbourne, Melbourne, Victoria 3052, Australia (D.V.I.).)

  • W. Zacheus Cande

    (Molecular and Cellular Biology, University of California Berkeley)

  • Francisco Antequera

    (Instituto de Biología Funcional y Genómica. CSIC/Universidad de Salamanca)

  • Benoit Arcangioli

    (Cold Spring Harbor Laboratory, Cold Spring Harbor
    Institut Pasteur)

  • Robert A. Martienssen

    (Cold Spring Harbor Laboratory, Cold Spring Harbor
    Watson School of Biological Sciences, Cold Spring Harbor Laboratory)

Abstract

RNAi keeps replication on track Transcription of heterochromatin during DNA replication regularly results in collision between DNA and RNA polymerases, and fork stalling can follow. Replication origins and silent heterochromatin, formed by RNA interference (RNAi), exhibit an alternating pattern near chromosome centromeres. Zaratiegui et al. show that RNAi displaces the RNA polymerase so that replication can be completed by the DNA polymerase. Histone-modifying enzymes travel with the replication fork, and in the absence of RNAi, stalled forks are repaired by recombination, but histone modifications are not made.

Suggested Citation

  • Mikel Zaratiegui & Stephane E. Castel & Danielle V. Irvine & Anna Kloc & Jie Ren & Fei Li & Elisa de Castro & Laura Marín & An-Yun Chang & Derek Goto & W. Zacheus Cande & Francisco Antequera & Benoit , 2011. "RNAi promotes heterochromatic silencing through replication-coupled release of RNA Pol II," Nature, Nature, vol. 479(7371), pages 135-138, November.
  • Handle: RePEc:nat:nature:v:479:y:2011:i:7371:d:10.1038_nature10501
    DOI: 10.1038/nature10501
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    Cited by:

    1. M. J. Gutbrod & B. Roche & J. I. Steinberg & A. A. Lakhani & K. Chang & A. J. Schorn & R. A. Martienssen, 2022. "Dicer promotes genome stability via the bromodomain transcriptional co-activator BRD4," Nature Communications, Nature, vol. 13(1), pages 1-14, December.

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