IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v479y2011i7371d10.1038_nature10501.html
   My bibliography  Save this article

RNAi promotes heterochromatic silencing through replication-coupled release of RNA Pol II

Author

Listed:
  • Mikel Zaratiegui

    (Cold Spring Harbor Laboratory, Cold Spring Harbor
    Present addresses: Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, New Jersey 08854, USA (M.Z.); Yale Stem Cell Center, Yale University, New Haven, Connecticut 06520, USA (A.K.); Creative Research Initiative Sousei, Hokkaido University, 001-0021 Sapporo, Japan (D.G.); Department of Biology, New York University, New York, New York 10003, USA (F.L.); Murdoch Children’s Research Institute, University of Melbourne, Melbourne, Victoria 3052, Australia (D.V.I.).)

  • Stephane E. Castel

    (Cold Spring Harbor Laboratory, Cold Spring Harbor
    Watson School of Biological Sciences, Cold Spring Harbor Laboratory)

  • Danielle V. Irvine

    (Cold Spring Harbor Laboratory, Cold Spring Harbor
    Present addresses: Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, New Jersey 08854, USA (M.Z.); Yale Stem Cell Center, Yale University, New Haven, Connecticut 06520, USA (A.K.); Creative Research Initiative Sousei, Hokkaido University, 001-0021 Sapporo, Japan (D.G.); Department of Biology, New York University, New York, New York 10003, USA (F.L.); Murdoch Children’s Research Institute, University of Melbourne, Melbourne, Victoria 3052, Australia (D.V.I.).)

  • Anna Kloc

    (Cold Spring Harbor Laboratory, Cold Spring Harbor
    Present addresses: Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, New Jersey 08854, USA (M.Z.); Yale Stem Cell Center, Yale University, New Haven, Connecticut 06520, USA (A.K.); Creative Research Initiative Sousei, Hokkaido University, 001-0021 Sapporo, Japan (D.G.); Department of Biology, New York University, New York, New York 10003, USA (F.L.); Murdoch Children’s Research Institute, University of Melbourne, Melbourne, Victoria 3052, Australia (D.V.I.).)

  • Jie Ren

    (Cold Spring Harbor Laboratory, Cold Spring Harbor)

  • Fei Li

    (Molecular and Cellular Biology, University of California Berkeley
    Present addresses: Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, New Jersey 08854, USA (M.Z.); Yale Stem Cell Center, Yale University, New Haven, Connecticut 06520, USA (A.K.); Creative Research Initiative Sousei, Hokkaido University, 001-0021 Sapporo, Japan (D.G.); Department of Biology, New York University, New York, New York 10003, USA (F.L.); Murdoch Children’s Research Institute, University of Melbourne, Melbourne, Victoria 3052, Australia (D.V.I.).)

  • Elisa de Castro

    (Instituto de Biología Funcional y Genómica. CSIC/Universidad de Salamanca)

  • Laura Marín

    (Instituto de Biología Funcional y Genómica. CSIC/Universidad de Salamanca)

  • An-Yun Chang

    (Cold Spring Harbor Laboratory, Cold Spring Harbor
    Molecular and Cell Biology program, Stony Brook University)

  • Derek Goto

    (Cold Spring Harbor Laboratory, Cold Spring Harbor
    Present addresses: Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, New Jersey 08854, USA (M.Z.); Yale Stem Cell Center, Yale University, New Haven, Connecticut 06520, USA (A.K.); Creative Research Initiative Sousei, Hokkaido University, 001-0021 Sapporo, Japan (D.G.); Department of Biology, New York University, New York, New York 10003, USA (F.L.); Murdoch Children’s Research Institute, University of Melbourne, Melbourne, Victoria 3052, Australia (D.V.I.).)

  • W. Zacheus Cande

    (Molecular and Cellular Biology, University of California Berkeley)

  • Francisco Antequera

    (Instituto de Biología Funcional y Genómica. CSIC/Universidad de Salamanca)

  • Benoit Arcangioli

    (Cold Spring Harbor Laboratory, Cold Spring Harbor
    Institut Pasteur)

  • Robert A. Martienssen

    (Cold Spring Harbor Laboratory, Cold Spring Harbor
    Watson School of Biological Sciences, Cold Spring Harbor Laboratory)

Abstract

RNAi keeps replication on track Transcription of heterochromatin during DNA replication regularly results in collision between DNA and RNA polymerases, and fork stalling can follow. Replication origins and silent heterochromatin, formed by RNA interference (RNAi), exhibit an alternating pattern near chromosome centromeres. Zaratiegui et al. show that RNAi displaces the RNA polymerase so that replication can be completed by the DNA polymerase. Histone-modifying enzymes travel with the replication fork, and in the absence of RNAi, stalled forks are repaired by recombination, but histone modifications are not made.

Suggested Citation

  • Mikel Zaratiegui & Stephane E. Castel & Danielle V. Irvine & Anna Kloc & Jie Ren & Fei Li & Elisa de Castro & Laura Marín & An-Yun Chang & Derek Goto & W. Zacheus Cande & Francisco Antequera & Benoit , 2011. "RNAi promotes heterochromatic silencing through replication-coupled release of RNA Pol II," Nature, Nature, vol. 479(7371), pages 135-138, November.
    • Handle: RePEc:nat:nature:v:479:y:2011:i:7371:d:10.1038_nature10501
    DOI: 10.1038/nature10501
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/nature10501
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/nature10501?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. M. J. Gutbrod & B. Roche & J. I. Steinberg & A. A. Lakhani & K. Chang & A. J. Schorn & R. A. Martienssen, 2022. "Dicer promotes genome stability via the bromodomain transcriptional co-activator BRD4," Nature Communications, Nature, vol. 13(1), pages 1-14, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:479:y:2011:i:7371:d:10.1038_nature10501. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.