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The role of Tet3 DNA dioxygenase in epigenetic reprogramming by oocytes

Author

Listed:
  • Tian-Peng Gu

    (Group of DNA Metabolism, The State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences)

  • Fan Guo

    (Group of DNA Metabolism, The State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences)

  • Hui Yang

    (The State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences)

  • Hai-Ping Wu

    (Group of DNA Metabolism, The State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
    Present address: Novartis Institutes for BioMedical Research Co., Shanghai 201203, China.)

  • Gui-Fang Xu

    (Group of DNA Metabolism, The State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences)

  • Wei Liu

    (Group of DNA Metabolism, The State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences)

  • Zhi-Guo Xie

    (Group of DNA Metabolism, The State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences)

  • Linyu Shi

    (The State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences)

  • Xinyi He

    (The State Key Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiaotong University)

  • Seung-gi Jin

    (Beckman Research Institute of the City of Hope)

  • Khursheed Iqbal

    (Beckman Research Institute of the City of Hope)

  • Yujiang Geno Shi

    (Diabetes, and Hypertension, Brigham and Women’s Hospital and Harvard Medical School)

  • Zixin Deng

    (The State Key Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiaotong University)

  • Piroska E. Szabó

    (Beckman Research Institute of the City of Hope)

  • Gerd P. Pfeifer

    (Beckman Research Institute of the City of Hope)

  • Jinsong Li

    (The State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences)

  • Guo-Liang Xu

    (Group of DNA Metabolism, The State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences)

Abstract

Oocyte reprogramming by Tet3 The paternal genome in a zygote undergoes active DNA demethylation before the fusion of the male and female pronuclei, and this coincides with oxidation of 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC). Here, Gu et al. generate conditional knockout mice lacking catalytic activity of the dioxygenase Tet3. In Tet3-deficient zygotes, conversion of 5mC into 5hmC in the paternal genome fails to occur and the level of 5mC remains constant. The demethylation of paternal Oct4 and Nanog genes is also impeded. Therefore, Tet3-mediated 5mC oxidation contributes to demethylation and gene activation in the zygotic paternal genome.

Suggested Citation

  • Tian-Peng Gu & Fan Guo & Hui Yang & Hai-Ping Wu & Gui-Fang Xu & Wei Liu & Zhi-Guo Xie & Linyu Shi & Xinyi He & Seung-gi Jin & Khursheed Iqbal & Yujiang Geno Shi & Zixin Deng & Piroska E. Szabó & Gerd , 2011. "The role of Tet3 DNA dioxygenase in epigenetic reprogramming by oocytes," Nature, Nature, vol. 477(7366), pages 606-610, September.
    • Handle: RePEc:nat:nature:v:477:y:2011:i:7366:d:10.1038_nature10443
    DOI: 10.1038/nature10443
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    Cited by:

    1. Francesca Pirini & Elisa Guida & Fahcina Lawson & Andrea Mancinelli & Rafael Guerrero-Preston, 2015. "Nuclear and Mitochondrial DNA Alterations in Newborns with Prenatal Exposure to Cigarette Smoke," IJERPH, MDPI, vol. 12(2), pages 1-21, January.
    2. Qing Li & Jiansen Lu & Xidi Yin & Yunjian Chang & Chao Wang & Meng Yan & Li Feng & Yanbo Cheng & Yun Gao & Beiying Xu & Yao Zhang & Yingyi Wang & Guizhong Cui & Luang Xu & Yidi Sun & Rong Zeng & Yixue, 2023. "Base editing-mediated one-step inactivation of the Dnmt gene family reveals critical roles of DNA methylation during mouse gastrulation," Nature Communications, Nature, vol. 14(1), pages 1-19, December.
    3. Jiajun Tan & Yingfeng Li & Xiang Li & Xiaoxiao Zhu & Liping Liu & Hua Huang & Jiahua Wei & Hailing Wang & Yong Tian & Zhigao Wang & Zhuqiang Zhang & Bing Zhu, 2024. "Pramel15 facilitates zygotic nuclear DNMT1 degradation and DNA demethylation," Nature Communications, Nature, vol. 15(1), pages 1-17, December.

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