IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v477y2011i7366d10.1038_nature10443.html
   My bibliography  Save this article

The role of Tet3 DNA dioxygenase in epigenetic reprogramming by oocytes

Author

Listed:
  • Tian-Peng Gu

    (Group of DNA Metabolism, The State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences)

  • Fan Guo

    (Group of DNA Metabolism, The State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences)

  • Hui Yang

    (The State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences)

  • Hai-Ping Wu

    (Group of DNA Metabolism, The State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
    Present address: Novartis Institutes for BioMedical Research Co., Shanghai 201203, China.)

  • Gui-Fang Xu

    (Group of DNA Metabolism, The State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences)

  • Wei Liu

    (Group of DNA Metabolism, The State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences)

  • Zhi-Guo Xie

    (Group of DNA Metabolism, The State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences)

  • Linyu Shi

    (The State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences)

  • Xinyi He

    (The State Key Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiaotong University)

  • Seung-gi Jin

    (Beckman Research Institute of the City of Hope)

  • Khursheed Iqbal

    (Beckman Research Institute of the City of Hope)

  • Yujiang Geno Shi

    (Diabetes, and Hypertension, Brigham and Women’s Hospital and Harvard Medical School)

  • Zixin Deng

    (The State Key Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiaotong University)

  • Piroska E. Szabó

    (Beckman Research Institute of the City of Hope)

  • Gerd P. Pfeifer

    (Beckman Research Institute of the City of Hope)

  • Jinsong Li

    (The State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences)

  • Guo-Liang Xu

    (Group of DNA Metabolism, The State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences)

Abstract

Oocyte reprogramming by Tet3 The paternal genome in a zygote undergoes active DNA demethylation before the fusion of the male and female pronuclei, and this coincides with oxidation of 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC). Here, Gu et al. generate conditional knockout mice lacking catalytic activity of the dioxygenase Tet3. In Tet3-deficient zygotes, conversion of 5mC into 5hmC in the paternal genome fails to occur and the level of 5mC remains constant. The demethylation of paternal Oct4 and Nanog genes is also impeded. Therefore, Tet3-mediated 5mC oxidation contributes to demethylation and gene activation in the zygotic paternal genome.

Suggested Citation

  • Tian-Peng Gu & Fan Guo & Hui Yang & Hai-Ping Wu & Gui-Fang Xu & Wei Liu & Zhi-Guo Xie & Linyu Shi & Xinyi He & Seung-gi Jin & Khursheed Iqbal & Yujiang Geno Shi & Zixin Deng & Piroska E. Szabó & Gerd , 2011. "The role of Tet3 DNA dioxygenase in epigenetic reprogramming by oocytes," Nature, Nature, vol. 477(7366), pages 606-610, September.
    • Handle: RePEc:nat:nature:v:477:y:2011:i:7366:d:10.1038_nature10443
    DOI: 10.1038/nature10443
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/nature10443
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/nature10443?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Qing Li & Jiansen Lu & Xidi Yin & Yunjian Chang & Chao Wang & Meng Yan & Li Feng & Yanbo Cheng & Yun Gao & Beiying Xu & Yao Zhang & Yingyi Wang & Guizhong Cui & Luang Xu & Yidi Sun & Rong Zeng & Yixue, 2023. "Base editing-mediated one-step inactivation of the Dnmt gene family reveals critical roles of DNA methylation during mouse gastrulation," Nature Communications, Nature, vol. 14(1), pages 1-19, December.
    2. Jiajun Tan & Yingfeng Li & Xiang Li & Xiaoxiao Zhu & Liping Liu & Hua Huang & Jiahua Wei & Hailing Wang & Yong Tian & Zhigao Wang & Zhuqiang Zhang & Bing Zhu, 2024. "Pramel15 facilitates zygotic nuclear DNMT1 degradation and DNA demethylation," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    3. Francesca Pirini & Elisa Guida & Fahcina Lawson & Andrea Mancinelli & Rafael Guerrero-Preston, 2015. "Nuclear and Mitochondrial DNA Alterations in Newborns with Prenatal Exposure to Cigarette Smoke," IJERPH, MDPI, vol. 12(2), pages 1-21, January.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:477:y:2011:i:7366:d:10.1038_nature10443. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.