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Condensin association with histone H2A shapes mitotic chromosomes

Author

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  • Kenji Tada

    (Laboratory of Chromosome Dynamics, Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi, Tokyo 113-0032, Japan
    Graduate Program in Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Yayoi, Tokyo 113-0032, Japan)

  • Hiroaki Susumu

    (Laboratory of Chromosome Dynamics, Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi, Tokyo 113-0032, Japan
    Graduate Program in Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Yayoi, Tokyo 113-0032, Japan)

  • Takeshi Sakuno

    (Laboratory of Chromosome Dynamics, Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi, Tokyo 113-0032, Japan
    Promotion of Independence for Young Investigators, University of Tokyo, Yayoi, Tokyo 113-0032, Japan)

  • Yoshinori Watanabe

    (Laboratory of Chromosome Dynamics, Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi, Tokyo 113-0032, Japan
    Graduate Program in Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Yayoi, Tokyo 113-0032, Japan)

Abstract

Chromosome structure is dynamically regulated during cell division, and this regulation is dependent, in part, on condensin. The localization of condensin at chromosome arms is crucial for chromosome partitioning during anaphase. Condensin is also enriched at kinetochores but its precise role and loading machinery remain unclear. Here we show that fission yeast (Schizosaccharomyces pombe) kinetochore proteins Pcs1 and Mde4—homologues of budding yeast (Saccharomyces cerevisiae) monopolin subunits and known to prevent merotelic kinetochore orientation—act as a condensin ‘recruiter’ at kinetochores, and that condensin itself may act to clamp microtubule binding sites during metaphase. In addition to the regional recruitment factors, overall condensin association with chromatin is governed by the chromosomal passenger kinase Aurora B. Aurora-B-dependent phosphorylation of condensin promotes its association with histone H2A and H2A.Z, which we identify as conserved chromatin ‘receptors’ of condensin. Condensin phosphorylation and its deposition onto chromosome arms reach a peak during anaphase, when Aurora B kinase relocates from centromeres to the spindle midzone, where the separating chromosome arms are positioned. Our results elucidate the molecular basis for the spatiotemporal regulation of mitotic chromosome architecture, which is crucial for chromosome partitioning.

Suggested Citation

  • Kenji Tada & Hiroaki Susumu & Takeshi Sakuno & Yoshinori Watanabe, 2011. "Condensin association with histone H2A shapes mitotic chromosomes," Nature, Nature, vol. 474(7352), pages 477-483, June.
    • Handle: RePEc:nat:nature:v:474:y:2011:i:7352:d:10.1038_nature10179
    DOI: 10.1038/nature10179
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    Cited by:

    1. Xi Wang & Fan Zheng & Yuan-yuan Yi & Gao-yuan Wang & Li-xin Hong & Dannel McCollum & Chuanhai Fu & Yamei Wang & Quan-wen Jin, 2022. "Ubiquitination of CLIP-170 family protein restrains polarized growth upon DNA replication stress," Nature Communications, Nature, vol. 13(1), pages 1-14, December.

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