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In vitro production of functional sperm in cultured neonatal mouse testes

Author

Listed:
  • Takuya Sato

    (Yokohama City University Graduate School of Medicine)

  • Kumiko Katagiri

    (Yokohama City University Graduate School of Medicine)

  • Ayako Gohbara

    (Yokohama City University Graduate School of Medicine)

  • Kimiko Inoue

    (RIKEN, Bioresource Center)

  • Narumi Ogonuki

    (RIKEN, Bioresource Center)

  • Atsuo Ogura

    (RIKEN, Bioresource Center)

  • Yoshinobu Kubota

    (Yokohama City University Graduate School of Medicine)

  • Takehiko Ogawa

    (Yokohama City University Graduate School of Medicine
    Project Leader of Advanced Medical Research Center, Yokohama City University)

Abstract

Preserving sperm fertility Reproducing the complex process of spermatogenesis in vitro might lead to the development of new diagnostic and therapeutic techniques for male infertility. Takehiko Ogawa and colleagues have now established in vitro organ culture conditions that can support the production of fertile sperm from spermatogonia of neonatal mice. Spermatids and sperm that were derived in vitro produced healthy and fertile mice. In addition, neonatal testis tissues that were cryopreserved for several months resumed complete spermatogenesis in vitro on thawing. The organ culture method is simple and, with further refinements, could be applicable to a variety of mammalian species. This work suggests that cryopreservation of the testis tissue of paediatric cancer patients could become a practical way of ensuring future fertility.

Suggested Citation

  • Takuya Sato & Kumiko Katagiri & Ayako Gohbara & Kimiko Inoue & Narumi Ogonuki & Atsuo Ogura & Yoshinobu Kubota & Takehiko Ogawa, 2011. "In vitro production of functional sperm in cultured neonatal mouse testes," Nature, Nature, vol. 471(7339), pages 504-507, March.
    • Handle: RePEc:nat:nature:v:471:y:2011:i:7339:d:10.1038_nature09850
    DOI: 10.1038/nature09850
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    Cited by:

    1. Ihsan Dereli & Vladyslav Telychko & Frantzeskos Papanikos & Kavya Raveendran & Jiaqi Xu & Michiel Boekhout & Marcello Stanzione & Benjamin Neuditschko & Naga Sailaja Imjeti & Elizaveta Selezneva & Has, 2024. "Seeding the meiotic DNA break machinery and initiating recombination on chromosome axes," Nature Communications, Nature, vol. 15(1), pages 1-23, December.

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