IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v470y2011i7335d10.1038_nature09726.html
   My bibliography  Save this article

Intrinsic transition of embryonic stem-cell differentiation into neural progenitors

Author

Listed:
  • Daisuke Kamiya

    (Organogenesis and Neurogenesis Group, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan
    Kyoto University Graduate School of Medicine, Kyoto 606-8501, Japan)

  • Satoe Banno

    (Organogenesis and Neurogenesis Group, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan)

  • Noriaki Sasai

    (Organogenesis and Neurogenesis Group, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan)

  • Masatoshi Ohgushi

    (Organogenesis and Neurogenesis Group, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan)

  • Hidehiko Inomata

    (Organogenesis and Neurogenesis Group, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan)

  • Kiichi Watanabe

    (Organogenesis and Neurogenesis Group, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan)

  • Masako Kawada

    (Organogenesis and Neurogenesis Group, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan)

  • Rieko Yakura

    (Organogenesis and Neurogenesis Group, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan)

  • Hiroshi Kiyonari

    (Laboratory of Animal Resource and Genetic Engineering, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan)

  • Kazuki Nakao

    (Laboratory of Animal Resource and Genetic Engineering, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan)

  • Lars Martin Jakt

    (Laboratory of Stem Cell Research, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan)

  • Shin-ichi Nishikawa

    (Laboratory of Stem Cell Research, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan)

  • Yoshiki Sasai

    (Organogenesis and Neurogenesis Group, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan
    Kyoto University Graduate School of Medicine, Kyoto 606-8501, Japan)

Abstract

The neural fate is generally considered to be the intrinsic direction of embryonic stem (ES) cell differentiation. However, little is known about the intracellular mechanism that leads undifferentiated cells to adopt the neural fate in the absence of extrinsic inductive signals. Here we show that the zinc-finger nuclear protein Zfp521 is essential and sufficient for driving the intrinsic neural differentiation of mouse ES cells. In the absence of the neural differentiation inhibitor BMP4, strong Zfp521 expression is intrinsically induced in differentiating ES cells. Forced expression of Zfp521 enables the neural conversion of ES cells even in the presence of BMP4. Conversely, in differentiation culture, Zfp521-depleted ES cells do not undergo neural conversion but tend to halt at the epiblast state. Zfp521 directly activates early neural genes by working with the co-activator p300. Thus, the transition of ES cell differentiation from the epiblast state into neuroectodermal progenitors specifically depends on the cell-intrinsic expression and activator function of Zfp521.

Suggested Citation

  • Daisuke Kamiya & Satoe Banno & Noriaki Sasai & Masatoshi Ohgushi & Hidehiko Inomata & Kiichi Watanabe & Masako Kawada & Rieko Yakura & Hiroshi Kiyonari & Kazuki Nakao & Lars Martin Jakt & Shin-ichi Ni, 2011. "Intrinsic transition of embryonic stem-cell differentiation into neural progenitors," Nature, Nature, vol. 470(7335), pages 503-509, February.
    • Handle: RePEc:nat:nature:v:470:y:2011:i:7335:d:10.1038_nature09726
    DOI: 10.1038/nature09726
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/nature09726
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/nature09726?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Anat Kreimer & Tal Ashuach & Fumitaka Inoue & Alex Khodaverdian & Chengyu Deng & Nir Yosef & Nadav Ahituv, 2022. "Massively parallel reporter perturbation assays uncover temporal regulatory architecture during neural differentiation," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    2. Balazs V. Varga & Maryam Faiz & Helena Pivonkova & Gabriel Khelifi & Huijuan Yang & Shangbang Gao & Emma Linderoth & Mei Zhen & Ragnhildur Thora Karadottir & Samer M. Hussein & Andras Nagy, 2022. "Signal requirement for cortical potential of transplantable human neuroepithelial stem cells," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    3. Luca Salerno & Carlo Cosentino & Giovanni Morrone & Francesco Amato, 2015. "Computational Modeling of a Transcriptional Switch Underlying B-Lymphocyte Lineage Commitment of Hematopoietic Multipotent Cells," PLOS ONE, Public Library of Science, vol. 10(7), pages 1-23, July.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:470:y:2011:i:7335:d:10.1038_nature09726. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.