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Crystal structure of bacterial RNA polymerase bound with a transcription inhibitor protein

Author

Listed:
  • Shunsuke Tagami

    (Graduate School of Science, University of Tokyo, 7-3-1 Hongo
    RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho)

  • Shun-ichi Sekine

    (Graduate School of Science, University of Tokyo, 7-3-1 Hongo
    RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho
    Laboratory of Structural Biology, Graduate School of Science, University of Tokyo, 7-3-1 Hongo)

  • Thirumananseri Kumarevel

    (Structural and Molecular Biology Laboratory, RIKEN SPring-8 Center, Harima Institute, 1-1-1 Kouto
    Present address: Biometal Science Laboratory, RIKEN SPring-8 Center, Harima Institute, 1-1-1 Kouto, Sayo, Hyogo 679-5148, Japan.)

  • Nobumasa Hino

    (RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho)

  • Yuko Murayama

    (Graduate School of Science, University of Tokyo, 7-3-1 Hongo
    RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho)

  • Syunsuke Kamegamori

    (Graduate School of Science, University of Tokyo, 7-3-1 Hongo
    RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho)

  • Masaki Yamamoto

    (SR Life Science Instrumentation Unit, RIKEN SPring-8 Center, Harima Institute, 1-1-1 Kouto)

  • Kensaku Sakamoto

    (RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho)

  • Shigeyuki Yokoyama

    (Graduate School of Science, University of Tokyo, 7-3-1 Hongo
    RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho
    Laboratory of Structural Biology, Graduate School of Science, University of Tokyo, 7-3-1 Hongo)

Abstract

RNA polymerase inhibition A crystal structure of bacterial RNA polymerase (RNAP) bound to the transcription inhibitor Gfh1 reveals the mechanism of inhibition by Gfh1 and shows RNAP in a novel 'ratcheted' conformation. The authors propose that the conformation they observe could be relevant for other stages of transcription such as translocation of the polymerase along DNA.

Suggested Citation

  • Shunsuke Tagami & Shun-ichi Sekine & Thirumananseri Kumarevel & Nobumasa Hino & Yuko Murayama & Syunsuke Kamegamori & Masaki Yamamoto & Kensaku Sakamoto & Shigeyuki Yokoyama, 2010. "Crystal structure of bacterial RNA polymerase bound with a transcription inhibitor protein," Nature, Nature, vol. 468(7326), pages 978-982, December.
    • Handle: RePEc:nat:nature:v:468:y:2010:i:7326:d:10.1038_nature09573
    DOI: 10.1038/nature09573
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    Cited by:

    1. Lin-Tai Da & Fátima Pardo Avila & Dong Wang & Xuhui Huang, 2013. "A Two-State Model for the Dynamics of the Pyrophosphate Ion Release in Bacterial RNA Polymerase," PLOS Computational Biology, Public Library of Science, vol. 9(4), pages 1-9, April.
    2. Simona Pilotto & Michal Sýkora & Gwenny Cackett & Christopher Dulson & Finn Werner, 2024. "Structure of the recombinant RNA polymerase from African Swine Fever Virus," Nature Communications, Nature, vol. 15(1), pages 1-15, December.

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