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U1 snRNP protects pre-mRNAs from premature cleavage and polyadenylation

Author

Listed:
  • Daisuke Kaida

    (Howard Hughes Medical Institute, University of Pennsylvania School of Medicine)

  • Michael G. Berg

    (Howard Hughes Medical Institute, University of Pennsylvania School of Medicine)

  • Ihab Younis

    (Howard Hughes Medical Institute, University of Pennsylvania School of Medicine)

  • Mumtaz Kasim

    (Howard Hughes Medical Institute, University of Pennsylvania School of Medicine)

  • Larry N. Singh

    (Howard Hughes Medical Institute, University of Pennsylvania School of Medicine)

  • Lili Wan

    (Howard Hughes Medical Institute, University of Pennsylvania School of Medicine)

  • Gideon Dreyfuss

    (Howard Hughes Medical Institute, University of Pennsylvania School of Medicine)

Abstract

In eukaryotes, U1 small nuclear ribonucleoprotein (snRNP) forms spliceosomes in equal stoichiometry with U2, U4, U5 and U6 snRNPs; however, its abundance in human far exceeds that of the other snRNPs. Here we used antisense morpholino oligonucleotide to U1 snRNA to achieve functional U1 snRNP knockdown in HeLa cells, and identified accumulated unspliced pre-mRNAs by genomic tiling microarrays. In addition to inhibiting splicing, U1 snRNP knockdown caused premature cleavage and polyadenylation in numerous pre-mRNAs at cryptic polyadenylation signals, frequently in introns near (

Suggested Citation

  • Daisuke Kaida & Michael G. Berg & Ihab Younis & Mumtaz Kasim & Larry N. Singh & Lili Wan & Gideon Dreyfuss, 2010. "U1 snRNP protects pre-mRNAs from premature cleavage and polyadenylation," Nature, Nature, vol. 468(7324), pages 664-668, December.
    • Handle: RePEc:nat:nature:v:468:y:2010:i:7324:d:10.1038_nature09479
    DOI: 10.1038/nature09479
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    Cited by:

    1. Ebru Aydin & Silke Schreiner & Jacqueline Böhme & Birte Keil & Jan Weber & Bojan Žunar & Timo Glatter & Cornelia Kilchert, 2024. "DEAD-box ATPase Dbp2 is the key enzyme in an mRNP assembly checkpoint at the 3’-end of genes and involved in the recycling of cleavage factors," Nature Communications, Nature, vol. 15(1), pages 1-20, December.
    2. Emily Kunce Stroup & Zhe Ji, 2023. "Deep learning of human polyadenylation sites at nucleotide resolution reveals molecular determinants of site usage and relevance in disease," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    3. Kenzui Taniue & Anzu Sugawara & Chao Zeng & Han Han & Xinyue Gao & Yuki Shimoura & Atsuko Nakanishi Ozeki & Rena Onoguchi-Mizutani & Masahide Seki & Yutaka Suzuki & Michiaki Hamada & Nobuyoshi Akimits, 2024. "The MTR4/hnRNPK complex surveils aberrant polyadenylated RNAs with multiple exons," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    4. Amy L. Hughes & Aleksander T. Szczurek & Jessica R. Kelley & Anna Lastuvkova & Anne H. Turberfield & Emilia Dimitrova & Neil P. Blackledge & Robert J. Klose, 2023. "A CpG island-encoded mechanism protects genes from premature transcription termination," Nature Communications, Nature, vol. 14(1), pages 1-19, December.

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